Lewis Typing of Human Bloodstains by Enzyme-Linked Immunosorbent Assay (ELISA) Using Monoclonal Anti-Lea and Anti-Leb

    Volume 32, Issue 4 (July 1987)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 6


    Terazawa, K
    Professor, research student, and associate professor, Hokkaido University School of Medicine, Sapporo,

    Takatori, T
    Professor, research student, and associate professor, Hokkaido University School of Medicine, Sapporo,

    Tustsubuchi, Y
    Professor, research student, and associate professor, Hokkaido University School of Medicine, Sapporo,

    (Received 30 August 1986; accepted 21 October 1986)

    Abstract

    The Lewis blood grouping of human dried bloodstains could be determined by an enzyme-linked immunosorbent assay (ELISA) using monoclonal anti-Lea and anti-Leb antibodies with an avidin-biotin complex (ABC). The bloodstains aged 1 year were used as samples, and approximately 1 mg of the stains was enough to type each Lewis antigen reliably by this method. The Lewis substances of 106 individual stains were correctly typed regardless of their ABO blood group system.


    Paper ID: JFS12401J

    DOI: 10.1520/JFS12401J

    ASTM International
    is a member of CrossRef.

    Author
    Title Lewis Typing of Human Bloodstains by Enzyme-Linked Immunosorbent Assay (ELISA) Using Monoclonal Anti-Lea and Anti-Leb
    Symposium , 0000-00-00
    Committee E30