Volume 32, Issue 4 (July 1987)

    Lewis Typing of Human Bloodstains by Enzyme-Linked Immunosorbent Assay (ELISA) Using Monoclonal Anti-Lea and Anti-Leb

    (Received 30 August 1986; accepted 21 October 1986)

    CODEN: JFSOAD

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    Abstract

    The Lewis blood grouping of human dried bloodstains could be determined by an enzyme-linked immunosorbent assay (ELISA) using monoclonal anti-Lea and anti-Leb antibodies with an avidin-biotin complex (ABC). The bloodstains aged 1 year were used as samples, and approximately 1 mg of the stains was enough to type each Lewis antigen reliably by this method. The Lewis substances of 106 individual stains were correctly typed regardless of their ABO blood group system.


    Author Information:

    Terazawa, K
    Professor, research student, and associate professor, Hokkaido University School of Medicine, Sapporo,

    Takatori, T
    Professor, research student, and associate professor, Hokkaido University School of Medicine, Sapporo,

    Tustsubuchi, Y
    Professor, research student, and associate professor, Hokkaido University School of Medicine, Sapporo,


    Stock #: JFS12401J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12401J

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    Title Lewis Typing of Human Bloodstains by Enzyme-Linked Immunosorbent Assay (ELISA) Using Monoclonal Anti-Lea and Anti-Leb
    Symposium , 0000-00-00
    Committee E30