Evaluation of the Abbott TDx-Radiative Energy Attenuation (REA) Ethanol Assay in a Study of 1105 Forensic Whole Blood Specimens

    Volume 32, Issue 1 (January 1987)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 7


    Levine, B
    Toxicologist and assistant toxicologist, Office of the Chief Medical Examiner, Baltimore, MD

    Caplan, YH
    Toxicologist and assistant toxicologist, Office of the Chief Medical Examiner, Baltimore, MD

    (Received 27 February 1986; accepted 1 May 1986)

    Abstract

    In a preliminary study to determine the applicability of the Abbott radiative energy attenuation (REA) method for the quantification of ethanol in whole blood specimens it was concluded that a larger number of samples was required to evaluate the method, particularly for use in forensic toxicology applications. In this study, 573 blood specimens from suspected driving while intoxicated individuals (DWI blood) and 532 postmortem blood specimens (PM blood) were analyzed by the REA method and a headspace gas chromatographic method (GC) currently used in this laboratory. “Negative” specimens (<10 mg/dL by GC) and “positive” specimens (≥ 10 mg/dL by GC) in each category were analyzed. Linear regression analysis comparing the REA values with the GC values was performed for each type of blood specimen. The equation obtained for DWI blood specimens was REA = 0.943 GC + 1.54; the equation for PM blood specimens was REA = 0.980 GC + 2.76. The correlation coefficient for each group was greater than 0.99. The data suggested that a limit of detection of 10 mg/dL could be applied for DWI blood specimens, while 20 mg/dL would be recommended as the limit of detection for PM blood specimens.


    Paper ID: JFS12326J

    DOI: 10.1520/JFS12326J

    ASTM International
    is a member of CrossRef.

    Author
    Title Evaluation of the Abbott TDx-Radiative Energy Attenuation (REA) Ethanol Assay in a Study of 1105 Forensic Whole Blood Specimens
    Symposium , 0000-00-00
    Committee E30