Screening for Barbiturates in Vitreous Humor by the EMIT®-st Serum Enzyme Immunoassay

    Volume 31, Issue 2 (April 1986)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 3


    Penttilä, A
    Assistant toxicologist, chief toxicologist, toxicologist, and associate professor and chief, University of Helsinki, Helsinki,

    Vuori, E
    Assistant toxicologist, chief toxicologist, toxicologist, and associate professor and chief, University of Helsinki, Helsinki,

    Ojanperä, I
    Assistant toxicologist, chief toxicologist, toxicologist, and associate professor and chief, University of Helsinki, Helsinki,

    Nieminen, R
    Assistant toxicologist, chief toxicologist, toxicologist, and associate professor and chief, University of Helsinki, Helsinki,

    (Received 13 June 1985; accepted 1 July 1985)

    Abstract

    In 16 medical examiner's cases, which were found to be barbiturate-positive by thin-layer chromatographic screening of the liver, blood barbiturate concentrations were determined by gas chromatography. The corresponding vitreous humor samples were screened by the enzyme multiplied immunoassay technique, the EMIT®-st serum barbiturate assay. By using the recommended dilution for detecting serum barbiturates, it was possible to detect barbiturates in vitreous humor at a toxic concentration. By using one fourth the amount of diluent, the barbiturates could be detected also at a therapeutic concentration. The EMIT-st assay proved to be useful in the screening for barbiturates in vitreous humor, a material that is readily available in forensic toxicology.


    Paper ID: JFS12303J

    DOI: 10.1520/JFS12303J

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    Title Screening for Barbiturates in Vitreous Humor by the EMIT®-st Serum Enzyme Immunoassay
    Symposium , 0000-00-00
    Committee E30