Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT®) d.a.u. and Roche Abuscreen® Radioimmunoassay (RIA) (<sup>125</sup>I) Urine Cannabinoid Immunoassays by Gas Chromatographic/Mass Spectrometric (GC/MS) and Bonded Phase Adsorption/Thin Layer Chromatographic (BPA TLC) Methods

Volume 31, Issue 2 (April 1986)

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ISSN: 0022-1198
CODEN: JFSCA
Page Count: 7

Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT®) d.a.u. and Roche Abuscreen® Radioimmunoassay (RIA) (¹²⁵I) Urine Cannabinoid Immunoassays by Gas Chromatographic/Mass Spectrometric (GC/MS) and Bonded-Phase Adsorption/Thin-Layer Chromatographic (BPA-TLC) Methods
Pierson, DJ
Head, Chromatography Section, head, Immunoassay Section, and associate laboratory director, New York State Psychiatric Institute, Department of Neuropathology and Neurotoxicology, NY

Al Razi, J
Head, Analytical Toxicology, Psychiatric Diagnostic Laboratories of America, Inc., NJ

Kogan, MJ
Head, Chromatography Section, head, Immunoassay Section, and associate laboratory director, New York State Psychiatric Institute, Department of Neuropathology and Neurotoxicology, NY

Willson, NJ
Head, Chromatography Section, head, Immunoassay Section, and associate laboratory director, New York State Psychiatric Institute, Department of Neuropathology and Neurotoxicology, NY

(Received 15 April 1985; accepted 9 August 1985)

Abstract

Thirty human urines screened positive by the Syva enzyme multiple immunoassay technique (EMIT®) d.a.u. urine cannabinoid assay were also positive for the major marijuana urinary metabolite 11-nor-Δ⁹-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) when assayed by gas chromatographic/mass spectrometric (GC/MS) and a noninstrumental qualitative bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) technique. The noninstrumental BPA-TLC procedure was the simpler of the two techniques to perform and interpret. Assay of these same samples by the Roche Abuscreen® radioimmunoassay (RIA) for cannabinoids (¹²⁵I) revealed that reliance on the 100-ng/mL equivalent positive calibrator yielded a high incidence of false negative results (10 out of 30). The performance of these same 4 assays on 30 true negatives also was evaluated. All samples were negative for cannabinoids by EMIT and RIA, and for THC-COOH by BPA-TLC. GC/MS assay, however, detected spurious low levels of approximately S-ng/mL THC-COOH in two instances. Because of this, a reliability level of 10 ng/mL was set for the routine quantitative confirmation of THC-COOH by the GC/MS method.

Keywords:
toxicology, immunoassay, marijuana, chromatographic analysis

Paper ID: JFS12280J
DOI: 10.1520/JFS12280J
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Author Title Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT®) d.a.u. and Roche Abuscreen® Radioimmunoassay (RIA) (¹²⁵I) Urine Cannabinoid Immunoassays by Gas Chromatographic/Mass Spectrometric (GC/MS) and Bonded-Phase Adsorption/Thin-Layer Chromatographic (BPA-TLC) Methods Symposium , 0000-00-00 Committee E30

		SEDL / Journals / Journal of Forensic Sciences (JOFS) / Citation Page Volume 31, Issue 2 (April 1986) Click here to download this paper now for $25 PDF (440K) View License Agreement ISSN: 0022-1198 CODEN: JFSCA Page Count: 7 Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT®) d.a.u. and Roche Abuscreen® Radioimmunoassay (RIA) (¹²⁵I) Urine Cannabinoid Immunoassays by Gas Chromatographic/Mass Spectrometric (GC/MS) and Bonded-Phase Adsorption/Thin-Layer Chromatographic (BPA-TLC) Methods Pierson, DJ Head, Chromatography Section, head, Immunoassay Section, and associate laboratory director, New York State Psychiatric Institute, Department of Neuropathology and Neurotoxicology, NY Al Razi, J Head, Analytical Toxicology, Psychiatric Diagnostic Laboratories of America, Inc., NJ Kogan, MJ Head, Chromatography Section, head, Immunoassay Section, and associate laboratory director, New York State Psychiatric Institute, Department of Neuropathology and Neurotoxicology, NY Willson, NJ Head, Chromatography Section, head, Immunoassay Section, and associate laboratory director, New York State Psychiatric Institute, Department of Neuropathology and Neurotoxicology, NY (Received 15 April 1985; accepted 9 August 1985) Abstract Thirty human urines screened positive by the Syva enzyme multiple immunoassay technique (EMIT®) d.a.u. urine cannabinoid assay were also positive for the major marijuana urinary metabolite 11-nor-Δ⁹-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) when assayed by gas chromatographic/mass spectrometric (GC/MS) and a noninstrumental qualitative bonded-phase adsorption/thin-layer chromatographic (BPA-TLC) technique. The noninstrumental BPA-TLC procedure was the simpler of the two techniques to perform and interpret. Assay of these same samples by the Roche Abuscreen® radioimmunoassay (RIA) for cannabinoids (¹²⁵I) revealed that reliance on the 100-ng/mL equivalent positive calibrator yielded a high incidence of false negative results (10 out of 30). The performance of these same 4 assays on 30 true negatives also was evaluated. All samples were negative for cannabinoids by EMIT and RIA, and for THC-COOH by BPA-TLC. GC/MS assay, however, detected spurious low levels of approximately S-ng/mL THC-COOH in two instances. Because of this, a reliability level of 10 ng/mL was set for the routine quantitative confirmation of THC-COOH by the GC/MS method. Keywords: toxicology, immunoassay, marijuana, chromatographic analysis Paper ID: JFS12280J DOI: 10.1520/JFS12280J ASTM International is a member of CrossRef. Author Title Confirmation of Syva Enzyme Multiple Immunoassay Technique (EMIT®) d.a.u. and Roche Abuscreen® Radioimmunoassay (RIA) (¹²⁵I) Urine Cannabinoid Immunoassays by Gas Chromatographic/Mass Spectrometric (GC/MS) and Bonded-Phase Adsorption/Thin-Layer Chromatographic (BPA-TLC) Methods Symposium , 0000-00-00 Committee E30