ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

A Microplate Method for Reverse ABO Typing of Bloodstains
Mudd, JL
Research chemist, FBI Academy FSRTC, VA

(Received 9 May 1985; accepted 3 September 1985)

Abstract

A sensitive and reliable hemagglutination assay, using V-bottom microplates, is described for the detection of the ABO blood group alloantibodies in bloodstained material. When used in conjunction with an absorption-elution procedure, the microplate assay resulted in a 300% increase in the number of conclusive grouping results when compared to the Lattes crust test. The use of the microplate reverse grouping assay permits 24 specimens to be assayed conveniently on a single plate and eliminates the tedious and time-consuming microscopic examination required for the Lattes crust test.

Keywords:
criminalistics, genetic typing, blood, alloantibodies, blood grouping, blood groups, hemagglutination, immunologic technics, and isoantibodies

Paper ID: JFS12271J
DOI: 10.1520/JFS12271J
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