Analysis of Restriction Fragment Length Polymorphisms in Deoxyribonucleic Acid (DNA) Recovered from Dried Bloodstains

    Volume 31, Issue 2 (April 1986)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 6


    Kanter, E
    Junior scientist, senior scientist, and laboratory director, Lifecodes Corp., Elmsford, NY

    Balazs, I
    Junior scientist, senior scientist, and laboratory director, Lifecodes Corp., Elmsford, NY

    Baird, M
    Junior scientist, senior scientist, and laboratory director, Lifecodes Corp., Elmsford, NY

    Shaler, R
    Director of serology, Office of the Chief Medical Examiner, New York, NY

    (Received 26 April 1985; accepted 12 August 1985)

    Abstract

    Deoxyribonucleic acid (DNA) was recovered from dried bloodstains aged up to three years and shown to be of high molecular weight. DNA was digested with restriction endonucleases and fractionated by agarose gel electrophoresis. Following transfer to a filter, DNA was hybridized with two different radioactively labeled recombinant probes which recognize highly polymorphic regions in human DNA. The autoradiographic pattern observed was not altered by sample age, and the size of the alleles was consistent with those observed in the general population. Therefore, DNA of high molecular weight prepared from dried blood samples can be used for identification.


    Paper ID: JFS12269J

    DOI: 10.1520/JFS12269J

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    Title Analysis of Restriction Fragment Length Polymorphisms in Deoxyribonucleic Acid (DNA) Recovered from Dried Bloodstains
    Symposium , 0000-00-00
    Committee E30