Journal Published Online: 01 October 1982
Volume 27, Issue 4

A Simple Spectrophotometry for Determination of Carboxyhemoglobin in Blood

CODEN: JFSCAS

Abstract

A simple spectrophotometric method for determination of carboxyhemoglobin (HbCO) in blood is described. Blood is dissolved in sodium carbonate solution at a dilution near 250-fold. The diluent contains sodium hydrosulfite providing the two-component system HbCO-reduced hemoglobin (HHb). The percentage of HbCO is calculated from absorbance values at 532 and 558 nm measured after the addition of sodium hydroxide, which makes the solution completely clear. Results obtained by the present method are in satisfactory agreement with those by the oxygen electrode method.

Author Information

Katsumata, Y
Nagoya University School of Medicine, Nagoya, Japan
Aoki, M
Nagoya University School of Medicine, Nagoya, Japan
Sato, K
Nagoya University School of Medicine, Nagoya, Japan
Suzuki, O
Hamamatsu University School of Medicine, Hamamatsu, Japan
Oya, M
Nagoya University School of Medicine, Nagoya, Japan
Yada, S
Nagoya University School of Medicine, Nagoya, Japan
Pages: 7
Price: $25.00
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Stock #: JFS12212J
ISSN: 0022-1198
DOI: 10.1520/JFS12212J