ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 October 1982
Page Count: 7

A Simple Spectrophotometry for Determination of Carboxyhemoglobin in Blood
Sato, K
Senior lecturer, forensic chemist, graduate student, assistant professor, and professor, Nagoya University School of Medicine,

Aoki, M
Senior lecturer, forensic chemist, graduate student, assistant professor, and professor, Nagoya University School of Medicine,

Oya, M
Senior lecturer, forensic chemist, graduate student, assistant professor, and professor, Nagoya University School of Medicine,

Suzuki, O
Assistant professor, Hamamatsu University School of Medicine,

Yada, S
Senior lecturer, forensic chemist, graduate student, assistant professor, and professor, Nagoya University School of Medicine,

Katsumata, Y
Senior lecturer, forensic chemist, graduate student, assistant professor, and professor, Nagoya University School of Medicine,

(Received 3 October 1981; accepted 29 January 1982)

Abstract

A simple spectrophotometric method for determination of carboxyhemoglobin (HbCO) in blood is described. Blood is dissolved in sodium carbonate solution at a dilution near 250-fold. The diluent contains sodium hydrosulfite providing the two-component system HbCO-reduced hemoglobin (HHb). The percentage of HbCO is calculated from absorbance values at 532 and 558 nm measured after the addition of sodium hydroxide, which makes the solution completely clear. Results obtained by the present method are in satisfactory agreement with those by the oxygen electrode method.

Keywords:
pathology and biology, spectroscopic analysis, carbon monoxide, carboxyhemoglobin

Paper ID: JFS12212J
DOI: 10.1520/JFS12212J
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