Journal Published Online: 01 October 1982
Volume 27, Issue 4

An Assessment of the Immunofluorescence Technique as a Method for Demonstrating the Histological Localization of Tetrahydrocannabinol in Mammalian Tissues

CODEN: JFSCAS

Abstract

The use of the indirect immunofluorescence technique as a method for demonstrating the histological localization of tetrahydrocannabinol (Δ-THC) has been examined. The experimental protocol was designed in order that optimal staining conditions with respect to temperature, the length of time of incubations and washes, and the dilution of the antisera should be defined. No marked differences were detected between frozen sections of liver from normal and Δ-THC-injected mice. Results from radiotracer experiments using human liver suggest that the success of the method is dependent upon the solubility characteristics of the antigen-antibody complex.

Author Information

Morley, M
University of Leeds, Leeds, United Kingdom
Gee, DJ
University of Leeds, Leeds, United Kingdom
Pages: 7
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Stock #: JFS12200J
ISSN: 0022-1198
DOI: 10.1520/JFS12200J