ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 October 1982
Page Count: 7

An Assessment of the Immunofluorescence Technique as a Method for Demonstrating the Histological Localization of Tetrahydrocannabinol in Mammalian Tissues
Gee, DJ
Research fellow and professor, University of Leeds,

Morley, M
Research fellow and professor, University of Leeds,

(Received 8 October 1981; accepted 20 January 1982)

Abstract

The use of the indirect immunofluorescence technique as a method for demonstrating the histological localization of tetrahydrocannabinol (Δ-THC) has been examined. The experimental protocol was designed in order that optimal staining conditions with respect to temperature, the length of time of incubations and washes, and the dilution of the antisera should be defined. No marked differences were detected between frozen sections of liver from normal and Δ-THC-injected mice. Results from radiotracer experiments using human liver suggest that the success of the method is dependent upon the solubility characteristics of the antigen-antibody complex.

Keywords:
pathology and biology, marihuana, histology, antigen-antibody reactions, radioactive isotopes, tetrahydrocannibinol, indirect immunofluorescence technique

Paper ID: JFS12200J
DOI: 10.1520/JFS12200J
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