Volume 27, Issue 3 (July 1982)
Determination of d-methamphetamine in Urine after Administration of d- or dl-methamphetamine to Rats by Radioimmunoassay Using Optically Sensitive Antiserum
A radioimmunoassay was developed for the determination of d-methamphetamine in urine. Antiserum to d-methamphetamine was prepared in rabbits by immunization with d-N-4-aminobutylmethamphetamine conjugated with bovine serum albumin, d-1-[3H]-Methamphetamine was used as a labeled compound for radioimmunoassay. The specificity of the antibody against d-methamphetamine was determined by cross-reaction studies with optical isomers of methamphetamine and its analogs. The antibody was specific for d-methamphetamine and exhibited no significant cross-reaction with thel-isomers. This stereoselective assay was applied to determination of d-methamphetamine excreted in urine after oral administration of d- or dl-methamphetamine to rats.