ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 1982
Page Count: 6

Determination of d-methamphetamine in Urine after Administration of d- or dl-methamphetamine to Rats by Radioimmunoassay Using Optically Sensitive Antiserum
Inoue, T
Chief, First Forensic Science Division,

Kishi, T
Chief, First Forensic Science Division,

Niwaguchi, T
Chief, First Forensic Science Division,

Kanda, Y
Chief, First Forensic Science Division,

(Received 29 October 1981; accepted 28 December 1981)

Abstract

A radioimmunoassay was developed for the determination of d-methamphetamine in urine. Antiserum to d-methamphetamine was prepared in rabbits by immunization with d-N-4-aminobutylmethamphetamine conjugated with bovine serum albumin, d-1-[³H]-Methamphetamine was used as a labeled compound for radioimmunoassay. The specificity of the antibody against d-methamphetamine was determined by cross-reaction studies with optical isomers of methamphetamine and its analogs. The antibody was specific for d-methamphetamine and exhibited no significant cross-reaction with thel-isomers. This stereoselective assay was applied to determination of d-methamphetamine excreted in urine after oral administration of d- or dl-methamphetamine to rats.

Keywords:
toxicology, d-methamphetamine, radioimmunoassay

Paper ID: JFS12171J
DOI: 10.1520/JFS12171J
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