Determination of d-methamphetamine in Urine after Administration of d- or dl-methamphetamine to Rats by Radioimmunoassay Using Optically Sensitive Antiserum

    Volume 27, Issue 3 (July 1982)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 1982

    Page Count: 6


    Inoue, T
    Chief, First Forensic Science Division, Tokyo,

    Kishi, T
    Chief, First Forensic Science Division, Tokyo,

    Niwaguchi, T
    Chief, First Forensic Science Division, Tokyo,

    Kanda, Y
    Chief, First Forensic Science Division, Tokyo,

    (Received 29 October 1981; accepted 28 December 1981)

    Abstract

    A radioimmunoassay was developed for the determination of d-methamphetamine in urine. Antiserum to d-methamphetamine was prepared in rabbits by immunization with d-N-4-aminobutylmethamphetamine conjugated with bovine serum albumin, d-1-[3H]-Methamphetamine was used as a labeled compound for radioimmunoassay. The specificity of the antibody against d-methamphetamine was determined by cross-reaction studies with optical isomers of methamphetamine and its analogs. The antibody was specific for d-methamphetamine and exhibited no significant cross-reaction with thel-isomers. This stereoselective assay was applied to determination of d-methamphetamine excreted in urine after oral administration of d- or dl-methamphetamine to rats.


    Paper ID: JFS12171J

    DOI: 10.1520/JFS12171J

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    Title Determination of d-methamphetamine in Urine after Administration of d- or dl-methamphetamine to Rats by Radioimmunoassay Using Optically Sensitive Antiserum
    Symposium , 0000-00-00
    Committee E30