Volume 25, Issue 2 (April 1980)

    A Comparison of Enzyme Immunoassay and Gas Chromatography/Mass Spectrometry in Forensic Toxicology

    (Received 21 April 1979; accepted 11 August 1979)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    A series of 137 urine samples were analyzed for drugs of abuse by enzyme immunoassay (EMIT® and by gas chromatography/mass spectrometry (GC/MS). Agreement between these methods was excellent and ranged from 93.4% for benzodiazepines to 98.5% for propoxyphene. EMIT false negatives were traced to the presence of elevated endogenous lysozyme or other interfering materials. In the case of moderate amounts of lysozyme the use of a blank would lead to correct results. Disagreement in the identification of nine benzodiazepine samples was found to be due to a low recovery of benzodiazepine metabolites from urine. Recovery could be improved by incubation of the urine sample with the enzyme/3-glucuronidase.


    Author Information:

    Bermes, EW
    Associate director of clinical laboratories, Loyola University Medical Center, Maywood, Ill.

    Fenton, J
    Director of chemistry, Crozer-Chester Medical Center, Upland, Chester, Pa.

    Schaffer, M
    Chief toxicologist and senior toxicologist, Fishbein Institute of Forensic Medicine, Chicago, Ill.

    Chen, NW
    Chief toxicologist and senior toxicologist, Fishbein Institute of Forensic Medicine, Chicago, Ill.


    Stock #: JFS12128J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12128J

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    Title A Comparison of Enzyme Immunoassay and Gas Chromatography/Mass Spectrometry in Forensic Toxicology
    Symposium , 0000-00-00
    Committee E30