Volume 44, Issue 5 (September 1999)

    Substitution of H2O for Formamide in the Sample Preparation Protocol for STR Analysis Using the Capillary Electrophoresis System: The Effects on Precision, Resolution, and Capillary Life

    (Received 24 July 1998; accepted 15 January 1999)

    CODEN: JFSOAD

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    Abstract

    Water has been evaluated as a suitable solvent to replace formamide in the preparation of samples for capillary electrophoresis analysis. Critical aspects relevant to forensic analysis were studied to test the validity of the substitution. Correct assignment of allele labels was reproducibly performed on known samples, and size determination was found to be precise. Three hundred injections, regardless the method of preparation, may be run on a single capillary without loss of precision or resolution.


    Author Information:

    Duceman, BW
    Forensic scientist II and director of biological science, New York State Police Forensic Investigation Center, Albany, NY

    Biega, LA
    Forensic scientist II and director of biological science, New York State Police Forensic Investigation Center, Albany, NY


    Stock #: JFS12034J

    ISSN: 0022-1198

    DOI: 10.1520/JFS12034J

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    Author
    Title Substitution of H2O for Formamide in the Sample Preparation Protocol for STR Analysis Using the Capillary Electrophoresis System: The Effects on Precision, Resolution, and Capillary Life
    Symposium , 0000-00-00
    Committee E30