ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

Substitution of H²O for Formamide in the Sample Preparation Protocol for STR Analysis Using the Capillary Electrophoresis System: The Effects on Precision, Resolution, and Capillary Life
Duceman, BW
Forensic scientist II and director of biological science, New York State Police Forensic Investigation Center, NY

Biega, LA
Forensic scientist II and director of biological science, New York State Police Forensic Investigation Center, NY

(Received 24 July 1998; accepted 15 January 1999)

Abstract

Water has been evaluated as a suitable solvent to replace formamide in the preparation of samples for capillary electrophoresis analysis. Critical aspects relevant to forensic analysis were studied to test the validity of the substitution. Correct assignment of allele labels was reproducibly performed on known samples, and size determination was found to be precise. Three hundred injections, regardless the method of preparation, may be run on a single capillary without loss of precision or resolution.

Keywords:
forensic science, short tandem repeats, DNA typing, capillary electrophoresis, resolution D3S1358, vWA, FGA, Amelogenin, THO1, TPOX, CSF1PO, D5S818, D13S317, and D7S820

Paper ID: JFS12034J
DOI: 10.1520/JFS12034J
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