Potassium Nitrite Reaction with 11-Nor-Delta9-Tetrahydrocannabinol-9-Carboxylic Acid in Urine in Relation to the Drug Screening Analysis

    Volume 44, Issue 5 (September 1999)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 5


    Tuinman, A
    Research Chemist, University of Tennessee, Knoxville,

    Lewis, LA
    Development Chemists, Analytical Services Organization, Lockheed Martin Energy Systems, Inc., Oak Ridge, TN

    Lewis, SA
    Development Chemists, Analytical Services Organization, Lockheed Martin Energy Systems, Inc., Oak Ridge, TN

    (Received 23 June 1998; accepted 19 January 1999)

    Abstract

    Recently potassium nitrite has been used as an adulterant to interfere with the analysis of 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in urine. A comprehensive study of the THC-COOH and nitrite reaction chemistry and stability under various conditions is presented. Reverse phase high performance liquid chromatography (HPLC) and negative electrospray mass spectrometry (ESMS) results are given to substantiate the derived reaction mechanism and properties leading to reaction termination. The addition of potassium carbonate as a buffering agent prior to or following sample void as a means of preventing the formation of a nitroso-complexed form of the 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid is evaluated.


    Paper ID: JFS12022J

    DOI: 10.1520/JFS12022J

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    Title Potassium Nitrite Reaction with 11-Nor-Delta9-Tetrahydrocannabinol-9-Carboxylic Acid in Urine in Relation to the Drug Screening Analysis
    Symposium , 0000-00-00
    Committee E30