Journal Published Online: 01 May 1992
Volume 37, Issue 3

Deoxyribonucleic Acid (DNA) Typing of Human Leukocyte Antigen (HLA)-DQA1 from Single Hairs in Japanese

CODEN: JFSCAS

Abstract

The deoxyribonucleic acid (DNA) typing of human leukocyte antigen (HLA)-DQA1 from single hairs is described. HLA-DQA1 genotypes could be determined from single plucked hair roots. However, it was not easy to type HLA-DQA1 with hair shaft portions. Increase in the specimens of hair shaft portions (over 10 cm in length) to get sufficient DNA caused inhibition of polymerase chain reaction (PCR). Synthetic melanin as well as the one extracted from hairs inhibited the PCR of the genomic DNA template when added to the PCR reaction at the concentrations over than 15 ng/100 µL. Therefore, typability of hair shaft portions seems to depend on the delicate balance of the concentrations of DNA and the contaminated melanin in the final DNA extracts.

Author Information

Uchihi, R
Nagoya University School of Medicine, Nagoya, Japan
Tamaki, K
Nagoya University School of Medicine, Nagoya, Japan
Kojima, T
Nagoya University School of Medicine, Nagoya, Japan
Yamamoto, T
Nagoya University School of Medicine, Nagoya, Japan
Katsumata, Y
Nagoya University School of Medicine, Nagoya, Japan
Pages: 7
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS11997J
ISSN: 0022-1198
DOI: 10.1520/JFS11997J