(Received 8 June 1987; accepted 29 June 1987)
Published Online: March
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A sensitive method for the detection of anti-A and anti-B hemagglutinins in fresh saliva has been developed. The method utilizes a bromelin treated erythrocyte suspension as indicator cells and includes a simple procedure to concentrate these hemagglutinins. Antiserum directed against immunoglobulin A enhances the hemagglutination assay. We find that these salivary hemagglutinins are present in over 90% of the population and that their titer remains stable over a period of two months. These hemagglutinins can be used to blood type the donor of a saliva sample and can be used in a confirmatory test that complements the commonly used absorption-inhibition test which is used to detect salivary blood group agglutinogens. In preliminary studies we have determined that hemagglutinins can be successfully isolated and analyzed from dried saliva stains.
Forensic scientist, New Jersey State Police North Regional Laboratory, Little Fall, NJ
Associate professor of biology and immunology, John Jay College of Criminal Justice C.U.N.Y., City University of New York, New York, NY
Stock #: JFS11952J