Volume 30, Issue 2 (April 1985)
The Analysis of Soil Samples by Reverse Phase-High Performance Liquid Chromatography Using Wavelength Ratioing
The technique of reverse phase high performance liquid chromatography (HPLC) is investigated for the forensic analysis of soils. Unlike in previous works on this subject, the method of detection of the peaks is modified to include detection at two different wavelengths in the ultraviolet and the ratio of the absorption intensities is determined and displayed. The results show that the chromatograms of all of the soil samples studied differ from each other quantitatively but not all can be differentiated qualitatively. It is concluded that this method of analysis is an excellent presumptive test but has not been proven to be individualizing. To increase the probative value of the test, another method of separation and perhaps detection must be chosen.