ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

Increasing the Sensitivity of Detection of the Electrophoretic Marker Group Specific Component in Agarose Gels by Double-Staining with Coomassie Brilliant Blue R250 and Silver
Budowle, B
Research chemist, Forensic Science Research Group, Laboratory Division, FBI Academy, VA

(Received 17 February 1984; accepted 2 April 1984)

Abstract

A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.

Keywords:
forensic science, group specific component, genetic typing, electrophoresis, bloodstain, Coomassie Brilliant Blue R250, silver stain, agarose gels

Paper ID: JFS11788J
DOI: 10.1520/JFS11788J
ASTM International is a member of CrossRef.