Volume 29, Issue 4 (October 1984)

    Increasing the Sensitivity of Detection of the Electrophoretic Marker Group Specific Component in Agarose Gels by Double-Staining with Coomassie Brilliant Blue R250 and Silver

    (Received 17 February 1984; accepted 2 April 1984)

    CODEN: JFSOAD

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    Abstract

    A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.


    Author Information:

    Budowle, B
    Research chemist, Forensic Science Research Group, Laboratory Division, FBI Academy, Quantico, VA


    Stock #: JFS11788J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11788J

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    Title Increasing the Sensitivity of Detection of the Electrophoretic Marker Group Specific Component in Agarose Gels by Double-Staining with Coomassie Brilliant Blue R250 and Silver
    Symposium , 0000-00-00
    Committee E30