Volume 29, Issue 4 (October 1984)
The Detection of Protein p30 in Seminal Stains by Means of Thin-Layer Immunoassay
The detection of p30 by means of an indirect thin-layer immunoassay (TIA) is described. Extracts from 20 samples can be analyzed in approximately 2 h with a detection limit of approximately 50 ng. The p30 protein was detected in seminal stains which had been stored at room temperature for six months and at 130°C for 4 h. Blood, saliva, urine, perspiration, and tears did not interefere with the method. The reliability of the method was demonstrated in a blind study.