ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

The Detection of Protein p30 in Seminal Stains by Means of Thin-Layer Immunoassay
Masibay, AS
Graduate student and associate professor, The George Washington University, DC

Lappas, NT
Graduate student and associate professor, The George Washington University, DC

(Received 12 November 1983; accepted 10 January 1984)

Abstract

The detection of p30 by means of an indirect thin-layer immunoassay (TIA) is described. Extracts from 20 samples can be analyzed in approximately 2 h with a detection limit of approximately 50 ng. The p30 protein was detected in seminal stains which had been stored at room temperature for six months and at 130°C for 4 h. Blood, saliva, urine, perspiration, and tears did not interefere with the method. The reliability of the method was demonstrated in a blind study.

Keywords:
pathology and biology, semen, proteins, immunoassay

Paper ID: JFS11786J
DOI: 10.1520/JFS11786J
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