(Received 13 October 1983; accepted 20 January 1984)
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The determination of sex from forcibly removed hairs in forensic science laboratories has, in the past, been based almost entirely on the presence or absence of the Y chromosome in the cells of the hair root sheath. Since the human male genotype is XY and the female is XX, a technique was devised that permits root sheath cells to be stained sequentially for the Y and then the X chromosome using quinacrine mustard. Following staining, the Y and the X chromosome fluorescence were observed, at pH 5.5 and 3.0, respectively, by epifluorescence. The X and Y chromosome counts obtained for a single hair root specimen were reported as a Y — X (Y minus X) score. The results reported show that specimens from males gave positive Y — X score while specimens from females gave negative Y — X scores. Results of an age study and blind trials were also reported.
Research chemist, FBI Academy, FBI Laboratory, Quantico, VA
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