ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

Identification of Human Blood with Hybridoma-Derived Antibody to Human Immunoglobulin G
Nishimukai, H
Professor, assistant, and associate professor, Medical College of Oita,

Kishida, T
Professor, assistant, and associate professor, Medical College of Oita,

Tamaki, Y
Professor, assistant, and associate professor, Medical College of Oita,

(Received 15 July 1983; accepted 15 September 1983)

Abstract

During production of monoclonal anti-Gamma (Gm) antibody by the hybridoma technique, an antihuman immunoglobulin G (IgG) antibody was obtained. Unlike conventional antihuman IgG heteroantisera, this antibody reacted with the serum of humans and chimpanzees but did not cross-react with that of other primates or lower animal species in hemagglutination-inhibition tests with anti-D-coated red cells. To examine for the practical utility of the antihuman IgG antibody in an enzyme-linked immunosorbent assay (ELISA) for identification of human blood, microtiter wells were coated with human IgG and allowed to react with the antibody in the presence of human or animal serum under test. The bound antibody was detected with enzyme labeled antimouse IgG. The ELISA gave satisfactory results.

Keywords:
pathology and biology, immunology, immunoglobulins, species identification, bloodstains, monoclonal antibodies, enzyme-linked immunosorbent assay

Paper ID: JFS11749J
DOI: 10.1520/JFS11749J
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