ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

A Medicolegal Study on Enzymic Fluorometry of Choline in Human Semen
Terazawa, K
Professor, research student, and lecturer, Hokkaido University School of Medicine,

Tomii, S
Professor, research student, and lecturer, Hokkaido University School of Medicine,

Takatori, T
Professor, research student, and lecturer, Hokkaido University School of Medicine,

(Received 11 April 1983; accepted 21 June 1983)

Abstract

Studies have been conducted on an enzymic fluorometric method based on an initial rate of reaction for the determination of choline. The reaction system consists of choline oxidase coupled to peroxidase and homovanillic acid. Concentrations of choline as low as 0.1 nmol could be detected by this procedure. The concentration of free choline in normal semen was 18.7 to 29.5 μmol/mL. Free choline in other body fluids was negligible. The cholinc concentrations in seminal stains maintained at room temperature were not changed during a 30-day period. Those concentrations in seminal fluids kept at room temperature were detected until at least the fifth day.

Keywords:
pathology and biology, choline, semen, sex, body fluid, enzymic fluorometry

Paper ID: JFS11713J
DOI: 10.1520/JFS11713J
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