Journal Published Online: 01 April 1984
Volume 29, Issue 2

A Medicolegal Study on Enzymic Fluorometry of Choline in Human Semen

CODEN: JFSCAS

Abstract

Studies have been conducted on an enzymic fluorometric method based on an initial rate of reaction for the determination of choline. The reaction system consists of choline oxidase coupled to peroxidase and homovanillic acid. Concentrations of choline as low as 0.1 nmol could be detected by this procedure. The concentration of free choline in normal semen was 18.7 to 29.5 μmol/mL. Free choline in other body fluids was negligible. The cholinc concentrations in seminal stains maintained at room temperature were not changed during a 30-day period. Those concentrations in seminal fluids kept at room temperature were detected until at least the fifth day.

Author Information

Takatori, T
Hokkaido University School of Medicine, Sapporo, Japan
Tomii, S
Hokkaido University School of Medicine, Sapporo, Japan
Terazawa, K
Hokkaido University School of Medicine, Sapporo, Japan
Pages: 6
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Stock #: JFS11713J
ISSN: 0022-1198
DOI: 10.1520/JFS11713J