Volume 29, Issue 2 (April 1984)

    A Medicolegal Study on Enzymic Fluorometry of Choline in Human Semen

    (Received 11 April 1983; accepted 21 June 1983)

    CODEN: JFSOAD

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    Abstract

    Studies have been conducted on an enzymic fluorometric method based on an initial rate of reaction for the determination of choline. The reaction system consists of choline oxidase coupled to peroxidase and homovanillic acid. Concentrations of choline as low as 0.1 nmol could be detected by this procedure. The concentration of free choline in normal semen was 18.7 to 29.5 μmol/mL. Free choline in other body fluids was negligible. The cholinc concentrations in seminal stains maintained at room temperature were not changed during a 30-day period. Those concentrations in seminal fluids kept at room temperature were detected until at least the fifth day.


    Author Information:

    Terazawa, K
    Professor, research student, and lecturer, Hokkaido University School of Medicine, Sapporo,

    Tomii, S
    Professor, research student, and lecturer, Hokkaido University School of Medicine, Sapporo,

    Takatori, T
    Professor, research student, and lecturer, Hokkaido University School of Medicine, Sapporo,


    Stock #: JFS11713J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11713J

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    Title A Medicolegal Study on Enzymic Fluorometry of Choline in Human Semen
    Symposium , 0000-00-00
    Committee E30