Liquid Chromatographic Analysis of Promethazine and Its Major Metabolites in Human Postmortem Material

    Volume 29, Issue 2 (April 1984)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 12


    Archer, AW
    Research chemist, Research Section, Department of Health New South Wales, Lidcombe,

    Allender, WJ
    Analyst, Forensic Toxicology Section, Department of Health New South Wales, Lidcombe, N.S.W.

    (Received 2 June 1983; accepted 12 August 1983)

    Abstract

    A method is described for the determination of promethazine and some of its major metabolites in postmortem specimens by enzymic digestion followed by high-pressure liquid chromatography of the extracts using a cyano-bonded (μ-Bondapak®-CN) silica column packing and a mobile phase consisting of methanol, water, and n-octylamine (adjusted to pH 8). The system will separate and quantitate promethazine sulphoxide, desmonomethyl promethazine, didesmethyl promethazine, and promethazine. Pericyazine (2-cyano-10-[3-(4-hydroxypiperidino)propyl] phenothiazine) was used as internal standard. The parent drug and metabolites were extracted from a enzyme digested tissue homogenates with ethyl acetate using a simple, single micro-extraction procedure. The method was applied to four cases of fatal poisoning involving promethazine ingestion.


    Paper ID: JFS11699J

    DOI: 10.1520/JFS11699J

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    Title Liquid Chromatographic Analysis of Promethazine and Its Major Metabolites in Human Postmortem Material
    Symposium , 0000-00-00
    Committee E30