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Volume 29, Issue 2 (April 1984)

ISSN: 0022-1198
CODEN: JFSCA
Page Count: 9


The Simultaneous Electrophoretic Analysis of Esterase D and Phosphoglucomutase Subtyping in Fresh Blood and in Dried Bloodstains
Kobilinksy, L
Assistant professor of biology and immunology, John Jay College of Criminal Justice, NY

Adamo, R
Research scientist, Department of Laboratories and Research, Forensic Science Laboratory, N.Y.

(Received 11 June 1983; accepted 26 July 1983)

Abstract

Phosphoglucomutase1 (PGM) subtyping and esterase D phenotyping were simultaneously performed by electrophoresis of bloodstained fibers using agarose and a Tris-maleic acid buffer system, pH 5.4. This method reduces anodal gel shrinkage and shortens development time when compared to the conventional electrophoretic technique for PGM subtyping which is performed at pH 7.4 using an agarose-starch substrate.



Keywords:
criminalistics, genetic typing, electrophoresis, phosphoglucomutase, esterase D

Paper ID: JFS11691J
DOI: 10.1520/JFS11691J
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Author Title The Simultaneous Electrophoretic Analysis of Esterase D and Phosphoglucomutase Subtyping in Fresh Blood and in Dried Bloodstains Symposium , 0000-00-00 Committee E30