High Performance Liquid Chromatography-Immunoassay of Δ9-Tetrahydrocannabinol and Its Metabolites in Urine

    Volume 29, Issue 1 (January 1984)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 10


    Johnson, JR
    Associate Director, Center for Human Toxicology University of Utah, Salt Lake City, UT

    Peat, MA
    Associate Director, Center for Human Toxicology University of Utah, Salt Lake City, UT

    Deyman, ME
    Associate Director, Center for Human Toxicology University of Utah, Salt Lake City, UT

    (Received 20 January 1983; accepted 8 April 1983)

    Abstract

    High performance liquid chromatographic-immunoassay (HPLC-IA) profiles of cannabinoid metabolites in urine samples were obtained using four different antisera. The urines were chromatographed on a reverse phase system using a gradient of acetonitrile in water (pH 3.3) and fractions collected every 30 s. Some urine samples were hydrolyzed with methanolic sodium hydroxide before fractionation. Peaks of immunoreactivity were detected at a fraction corresponding to 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (COOH-THC) and at an early eluting fraction; however, the profiles depended upon the specificity of the antisera used.


    Paper ID: JFS11641J

    DOI: 10.1520/JFS11641J

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    Title High Performance Liquid Chromatography-Immunoassay of Δ9-Tetrahydrocannabinol and Its Metabolites in Urine
    Symposium , 0000-00-00
    Committee E30