Volume 29, Issue 1 (January 1984)

    High Performance Liquid Chromatography-Immunoassay of Δ9-Tetrahydrocannabinol and Its Metabolites in Urine

    (Received 20 January 1983; accepted 8 April 1983)

    CODEN: JFSOAD

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    Abstract

    High performance liquid chromatographic-immunoassay (HPLC-IA) profiles of cannabinoid metabolites in urine samples were obtained using four different antisera. The urines were chromatographed on a reverse phase system using a gradient of acetonitrile in water (pH 3.3) and fractions collected every 30 s. Some urine samples were hydrolyzed with methanolic sodium hydroxide before fractionation. Peaks of immunoreactivity were detected at a fraction corresponding to 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (COOH-THC) and at an early eluting fraction; however, the profiles depended upon the specificity of the antisera used.


    Author Information:

    Johnson, JR
    Associate Director, Center for Human Toxicology University of Utah, Salt Lake City, UT

    Peat, MA
    Associate Director, Center for Human Toxicology University of Utah, Salt Lake City, UT

    Deyman, ME
    Associate Director, Center for Human Toxicology University of Utah, Salt Lake City, UT


    Stock #: JFS11641J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11641J

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    Author
    Title High Performance Liquid Chromatography-Immunoassay of Δ9-Tetrahydrocannabinol and Its Metabolites in Urine
    Symposium , 0000-00-00
    Committee E30