ISSN: 0022-1198
CODEN: JFSCA
Page Count: 10

High Performance Liquid Chromatography-Immunoassay of Δ⁹-Tetrahydrocannabinol and Its Metabolites in Urine
Johnson, JR
Associate Director, Center for Human Toxicology University of Utah, UT

Peat, MA
Associate Director, Center for Human Toxicology University of Utah, UT

Deyman, ME
Associate Director, Center for Human Toxicology University of Utah, UT

(Received 20 January 1983; accepted 8 April 1983)

Abstract

High performance liquid chromatographic-immunoassay (HPLC-IA) profiles of cannabinoid metabolites in urine samples were obtained using four different antisera. The urines were chromatographed on a reverse phase system using a gradient of acetonitrile in water (pH 3.3) and fractions collected every 30 s. Some urine samples were hydrolyzed with methanolic sodium hydroxide before fractionation. Peaks of immunoreactivity were detected at a fraction corresponding to 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (COOH-THC) and at an early eluting fraction; however, the profiles depended upon the specificity of the antisera used.

Keywords:
toxicology, chromatographic analysis, immunoassay, tetrahydrocannabinol, urine

Paper ID: JFS11641J
DOI: 10.1520/JFS11641J
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