Journal Published Online: 01 January 1984
Volume 29, Issue 1

High Performance Liquid Chromatography-Immunoassay of Δ -Tetrahydrocannabinol and Its Metabolites in Urine

CODEN: JFSCAS

Abstract

High performance liquid chromatographic-immunoassay (HPLC-IA) profiles of cannabinoid metabolites in urine samples were obtained using four different antisera. The urines were chromatographed on a reverse phase system using a gradient of acetonitrile in water (pH 3.3) and fractions collected every 30 s. Some urine samples were hydrolyzed with methanolic sodium hydroxide before fractionation. Peaks of immunoreactivity were detected at a fraction corresponding to 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (COOH-THC) and at an early eluting fraction; however, the profiles depended upon the specificity of the antisera used.

Author Information

Peat, MA
Center for Human Toxicology University of Utah, Salt Lake City, UT
Deyman, ME
Center for Human Toxicology University of Utah, Salt Lake City, UT
Johnson, JR
Center for Human Toxicology University of Utah, Salt Lake City, UT
Pages: 10
Price: $25.00
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Stock #: JFS11641J
ISSN: 0022-1198
DOI: 10.1520/JFS11641J