Journal Published Online: 01 January 1984
Volume 29, Issue 1

Species Identification of Blood and Saliva Stains by Enzyme-Linked Immunoassay (ELISA) Using Monoclonal Antibody

CODEN: JFSCAS

Abstract

An indirect enzyme-linked immunoassay (ELISA) method for the identification of human blood and saliva stains is reported. The method uses a monoclonal antibody which reacts with human immunoglobulin G (IgG) in extracts of blood and saliva stains up to 16 months old. Semen stain extracts gave weak or negative results. For routine screening purposes dilutions of 1:1000 for bloodstain extracts and 1:100 for saliva stain extracts would be suitable. Of 32 other animal species tested, only chimpanzee, mouse, rat, and eel cross-reacted significantly, and the presence of the last three was clearly indicated by appropriate controls. The monoclonal antibody gave poor results in the crossover and gel diffusion techniques.

Author Information

Fletcher, SM
Central Research Establishment Home Office Forensic Science Service Aldermaston, Reading, Berkshire, England
Dolton, P
Central Research Establishment Home Office Forensic Science Service Aldermaston, Reading, Berkshire, England
Harris-Smith, PW
Central Research Establishment Home Office Forensic Science Service Aldermaston, Reading, Berkshire, England
Pages: 8
Price: $25.00
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Stock #: JFS11635J
ISSN: 0022-1198
DOI: 10.1520/JFS11635J