Volume 29, Issue 1 (January 1984)

    Species Identification of Blood and Saliva Stains by Enzyme-Linked Immunoassay (ELISA) Using Monoclonal Antibody

    (Received 4 February 1983; accepted 29 April 1983)

    CODEN: JFSOAD

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    Abstract

    An indirect enzyme-linked immunoassay (ELISA) method for the identification of human blood and saliva stains is reported. The method uses a monoclonal antibody which reacts with human immunoglobulin G (IgG) in extracts of blood and saliva stains up to 16 months old. Semen stain extracts gave weak or negative results. For routine screening purposes dilutions of 1:1000 for bloodstain extracts and 1:100 for saliva stain extracts would be suitable. Of 32 other animal species tested, only chimpanzee, mouse, rat, and eel cross-reacted significantly, and the presence of the last three was clearly indicated by appropriate controls. The monoclonal antibody gave poor results in the crossover and gel diffusion techniques.


    Author Information:

    Harris-Smith, PW
    Senior scientific officer, scientific officer, and higher scientific officer, Central Research Establishment Home Office Forensic Science Service Aldermaston, Reading, Berkshire,

    Dolton, P
    Senior scientific officer, scientific officer, and higher scientific officer, Central Research Establishment Home Office Forensic Science Service Aldermaston, Reading, Berkshire,

    Fletcher, SM
    Senior scientific officer, scientific officer, and higher scientific officer, Central Research Establishment Home Office Forensic Science Service Aldermaston, Reading, Berkshire,


    Stock #: JFS11635J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11635J

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    Title Species Identification of Blood and Saliva Stains by Enzyme-Linked Immunoassay (ELISA) Using Monoclonal Antibody
    Symposium , 0000-00-00
    Committee E30