Published Online: 1 October 1983
Page Count: 7
Forensic chemist, Drug Enforcement Administration, Special Testing and Research Laboratory, McLean, VA
Forensic chemist, Drug Enforcement Administration, Northeast Laboratory, New York, NY
(Received 9 December 1982; accepted 28 February 1983)
A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, and phosphoric acid mobile phase and a bonded cyano-amino-type polar phase column has been developed for the rapid, selective, and accurate quantitation of psilocybin in dry mushroom material. A simple one-step procedure is used for the quantitative extraction of psilocybin in under 60 min. The 267:254 nm absorbance ratio is used as a check on peak purity for the psilocybin response.
Paper ID: JFS11601J