(Received 9 December 1982; accepted 28 February 1983)
Published Online: October
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A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, and phosphoric acid mobile phase and a bonded cyano-amino-type polar phase column has been developed for the rapid, selective, and accurate quantitation of psilocybin in dry mushroom material. A simple one-step procedure is used for the quantitative extraction of psilocybin in under 60 min. The 267:254 nm absorbance ratio is used as a check on peak purity for the psilocybin response.
Forensic chemist, Drug Enforcement Administration, Special Testing and Research Laboratory, McLean, VA
Forensic chemist, Drug Enforcement Administration, Northeast Laboratory, New York, NY
Stock #: JFS11601J