Chromophoric Labeling of Cannabinoids with 4-Dimethylaminoazobenzene-4'-Sulfonyl Chloride

    Volume 28, Issue 4 (October 1983)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 October 1983

    Page Count: 11


    Maseda, C
    Research scientist, Research Laboratory of Scientific Investigation, Shimane Prefectural Police Headquarters, Matsue,

    Kimura, K
    Professor and associate technical staffs, Shimane Medical University, Izumo,

    Matsubara, K
    Professor and associate technical staffs, Shimane Medical University, Izumo,

    Fukui, Y
    Professor and associate technical staffs, Shimane Medical University, Izumo,

    (Received 27 October 1982; accepted 8 March 1983)

    Abstract

    A simple and sensitive assay for the cannabinoids is presented using a dabsylation procedure. Dabsyl derivatives of Δ9 -tetrahydrocannabinol (Δ9 -THC) and cannabinol (CBN) were prepared by reacting with 4-dimethylaminoazobenzene-4'-sulfonyl chloride (dabsyl chloride) in acetone in the presence of sodium carbonate-sodium bicarbonate buffer (pH 10). Crystalline dabsylcannabinoids gave intense absorption in the visible region. With these derivatives, analysis by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) were tested. These techniques gave good separation and nanogram detection of dabsyl-THC and -CBN by using n-hexane-ethyl acetate-diethylamine (20: 5: 1) for TLC and MeOH—H20 (95 : 5) at 450 nm for HPLC.


    Paper ID: JFS11599J

    DOI: 10.1520/JFS11599J

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    Title Chromophoric Labeling of Cannabinoids with 4-Dimethylaminoazobenzene-4'-Sulfonyl Chloride
    Symposium , 0000-00-00
    Committee E30