Volume 28, Issue 4 (October 1983)
Chromophoric Labeling of Cannabinoids with 4-Dimethylaminoazobenzene-4'-Sulfonyl Chloride
A simple and sensitive assay for the cannabinoids is presented using a dabsylation procedure. Dabsyl derivatives of Δ9 -tetrahydrocannabinol (Δ9 -THC) and cannabinol (CBN) were prepared by reacting with 4-dimethylaminoazobenzene-4'-sulfonyl chloride (dabsyl chloride) in acetone in the presence of sodium carbonate-sodium bicarbonate buffer (pH 10). Crystalline dabsylcannabinoids gave intense absorption in the visible region. With these derivatives, analysis by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) were tested. These techniques gave good separation and nanogram detection of dabsyl-THC and -CBN by using n-hexane-ethyl acetate-diethylamine (20: 5: 1) for TLC and MeOH—H20 (95 : 5) at 450 nm for HPLC.