A Rapid Isolation Technique for Drugs from Tissues and Fluids: Use of the Du Pont Prep 1 System

    Volume 27, Issue 1 (January 1982)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1982

    Page Count: 9


    Donnelly, B
    Assistant toxicologist, Long Island Jewish-Hillside Medical Center, Glen Oaks, Queens, NY

    Prendes, D
    Assistant professor of pharmacology and toxicology, School of Pharmacy, and graduate student, St. Johns University, Jamaica, Queens, NY

    Balkon, J
    Assistant professor of pharmacology and toxicology, School of Pharmacy, and graduate student, St. Johns University, Jamaica, Queens, NY

    (Received 6 March 1981; accepted 22 May 1981)

    Abstract

    A microprocessor-controlled, automated extraction/concentration device, the Prep 1 (Du Pont Clinical Systems), was evaluated for application to the isolation of drug substances from postmortem fluids and tissue homogenates. Two classes of materials were investigated: barbiturates and the benzodiazepine, diazepam. With as little as 200 mg of tissue, barbiturate derivatives were successfully isolated and measured by gas chromatography using nitrogen-phosphorus detection with a coefficient of variation of 2 to 5%. Diazepam was measured in a similar fashion with a coefficient of variation of 4.4%. Preliminary investigation indicates that this system is applicable to a wide range of drug substances of toxicological interest.


    Paper ID: JFS11447J

    DOI: 10.1520/JFS11447J

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    Title A Rapid Isolation Technique for Drugs from Tissues and Fluids: Use of the Du Pont Prep 1 System
    Symposium , 0000-00-00
    Committee E30