ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 1982
Page Count: 9

A Rapid Isolation Technique for Drugs from Tissues and Fluids: Use of the Du Pont Prep 1 System
Donnelly, B
Assistant toxicologist, Long Island Jewish-Hillside Medical Center, NY

Prendes, D
Assistant professor of pharmacology and toxicology, School of Pharmacy, and graduate student, St. Johns University, NY

Balkon, J
Assistant professor of pharmacology and toxicology, School of Pharmacy, and graduate student, St. Johns University, NY

(Received 6 March 1981; accepted 22 May 1981)

Abstract

A microprocessor-controlled, automated extraction/concentration device, the Prep 1 (Du Pont Clinical Systems), was evaluated for application to the isolation of drug substances from postmortem fluids and tissue homogenates. Two classes of materials were investigated: barbiturates and the benzodiazepine, diazepam. With as little as 200 mg of tissue, barbiturate derivatives were successfully isolated and measured by gas chromatography using nitrogen-phosphorus detection with a coefficient of variation of 2 to 5%. Diazepam was measured in a similar fashion with a coefficient of variation of 4.4%. Preliminary investigation indicates that this system is applicable to a wide range of drug substances of toxicological interest.

Keywords:
toxicology, drug identification

Paper ID: JFS11447J
DOI: 10.1520/JFS11447J
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