A New Enzymatic Method for the Demonstration of Choline in Human Seminal Stains

    Volume 26, Issue 2 (April 1981)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 April 1981

    Page Count: 6


    Asano, M
    Senior lecturer and professor (director), Hamamatsu University School of Medicine, Hamamatsu,

    Oya, M
    Associate professor and senior lecturer, Nagoya University School of Medicine, Nagoya,

    Suzuki, O
    Senior lecturer and professor (director), Hamamatsu University School of Medicine, Hamamatsu,

    Matsumoto, T
    Research associate, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate professor and senior lecturer, Nagoya University School of Medicine, Nagoya,

    (Received 14 August 1980; accepted 8 October 1980)

    Abstract

    A new, simple method for the identification of seminal stains based on the reaction of choline oxidase with choline is presented. The key reaction is the production of hydrogen peroxide by the oxidase action on choline, whereby hydrogen peroxide reacts with N-ethyl-N-(3-methylphenyl)-N'-acetylethylenediamine and 4-aminoantipyrine (ampyrone) in the presence of peroxidase to produce a purple color so that seminal fluid can be identified. Some data are presented on the sensitivity, stability, and specificity of the present method.


    Paper ID: JFS11377J

    DOI: 10.1520/JFS11377J

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    Title A New Enzymatic Method for the Demonstration of Choline in Human Seminal Stains
    Symposium , 0000-00-00
    Committee E30