ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 April 1981
Page Count: 6

A New Enzymatic Method for the Demonstration of Choline in Human Seminal Stains
Asano, M
Senior lecturer and professor (director), Hamamatsu University School of Medicine,

Oya, M
Associate professor and senior lecturer, Nagoya University School of Medicine,

Suzuki, O
Senior lecturer and professor (director), Hamamatsu University School of Medicine,

Matsumoto, T
Research associate, Nagoya University School of Medicine,

Katsumata, Y
Associate professor and senior lecturer, Nagoya University School of Medicine,

(Received 14 August 1980; accepted 8 October 1980)

Abstract

A new, simple method for the identification of seminal stains based on the reaction of choline oxidase with choline is presented. The key reaction is the production of hydrogen peroxide by the oxidase action on choline, whereby hydrogen peroxide reacts with N-ethyl-N-(3-methylphenyl)-N'-acetylethylenediamine and 4-aminoantipyrine (ampyrone) in the presence of peroxidase to produce a purple color so that seminal fluid can be identified. Some data are presented on the sensitivity, stability, and specificity of the present method.

Keywords:
pathology and biology, semen, choline, chemical analysis

Paper ID: JFS11377J
DOI: 10.1520/JFS11377J
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