Volume 26, Issue 2 (April 1981)

    A New Enzymatic Method for the Demonstration of Choline in Human Seminal Stains

    (Received 14 August 1980; accepted 8 October 1980)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    A new, simple method for the identification of seminal stains based on the reaction of choline oxidase with choline is presented. The key reaction is the production of hydrogen peroxide by the oxidase action on choline, whereby hydrogen peroxide reacts with N-ethyl-N-(3-methylphenyl)-N'-acetylethylenediamine and 4-aminoantipyrine (ampyrone) in the presence of peroxidase to produce a purple color so that seminal fluid can be identified. Some data are presented on the sensitivity, stability, and specificity of the present method.


    Author Information:

    Asano, M
    Senior lecturer and professor (director), Hamamatsu University School of Medicine, Hamamatsu,

    Oya, M
    Associate professor and senior lecturer, Nagoya University School of Medicine, Nagoya,

    Suzuki, O
    Senior lecturer and professor (director), Hamamatsu University School of Medicine, Hamamatsu,

    Matsumoto, T
    Research associate, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate professor and senior lecturer, Nagoya University School of Medicine, Nagoya,


    Stock #: JFS11377J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11377J

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    Author
    Title A New Enzymatic Method for the Demonstration of Choline in Human Seminal Stains
    Symposium , 0000-00-00
    Committee E30