Journal Published Online: 01 April 1981
Volume 26, Issue 2

Isoelectric Focusing in Agarose: Phosphoglucomutase (PGM Locus 1) Typing

CODEN: JFSCAS

Abstract

The subphenotypes of phosphoglucomutase (PGM) were successfully typed by isoelectric focusing with a charge-balanced agarose. The resolution was as high as that obtained with polyacrylamide, with none of the drawbacks associated with the use of polyacrylamide. Exceptionally straight iso-pH lines were obtained, making interpretation of the results more reliable. Gel preparation was simple and rapid, and the time for analysis was reduced. A way of preserving the resultant zymogram is described.

Author Information

Burdett, PE
Pharmacia Fine Chemicals AB, Uppsala 1, Sweden
Pages: 5
Price: $25.00
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Stock #: JFS11376J
ISSN: 0022-1198
DOI: 10.1520/JFS11376J