Volume 26, Issue 2 (April 1981)

    Isoelectric Focusing in Agarose: Phosphoglucomutase (PGM Locus 1) Typing

    (Received 14 August 1980; accepted 8 October 1980)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    The subphenotypes of phosphoglucomutase (PGM) were successfully typed by isoelectric focusing with a charge-balanced agarose. The resolution was as high as that obtained with polyacrylamide, with none of the drawbacks associated with the use of polyacrylamide. Exceptionally straight iso-pH lines were obtained, making interpretation of the results more reliable. Gel preparation was simple and rapid, and the time for analysis was reduced. A way of preserving the resultant zymogram is described.


    Author Information:

    Burdett, PE
    Product specialist, Pharmacia Fine Chemicals AB, Uppsala 1,


    Stock #: JFS11376J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11376J

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    Author
    Title Isoelectric Focusing in Agarose: Phosphoglucomutase (PGM Locus 1) Typing
    Symposium , 0000-00-00
    Committee E30