ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 April 1981
Page Count: 5

Isoelectric Focusing in Agarose: Phosphoglucomutase (PGM Locus 1) Typing
Burdett, PE
Product specialist, Pharmacia Fine Chemicals AB,

(Received 14 August 1980; accepted 8 October 1980)

Abstract

The subphenotypes of phosphoglucomutase (PGM) were successfully typed by isoelectric focusing with a charge-balanced agarose. The resolution was as high as that obtained with polyacrylamide, with none of the drawbacks associated with the use of polyacrylamide. Exceptionally straight iso-pH lines were obtained, making interpretation of the results more reliable. Gel preparation was simple and rapid, and the time for analysis was reduced. A way of preserving the resultant zymogram is described.

Keywords:
pathology and biology, genetic typing, phosphoglucomutase, isoelectric focusing

Paper ID: JFS11376J
DOI: 10.1520/JFS11376J
ASTM International is a member of CrossRef.