Journal Published Online: 01 January 1981
Volume 26, Issue 1

Simultaneous Determination of Nicotine and Cotinine in Human Plasma by Nitrogen Detection Gas-Liquid Chromatography

CODEN: JFSCAS

Abstract

Human plasma levels of nicotine and its principal metabolite, cotinine, were simultaneously quantitated by gas-liquid chromatography combined with nitrogen selective detection. Nicotine, cotinine, and the added internal standard ketamine are extracted from plasma at basic pH into methylene chloride, back-extracted into acid, and then re-extracted into methylene chloride. Analysis is carried out on a packed glass column of 3% SE-30 while column temperature is programmed from 150 to 200°C. Detector response is linear over the range of 2 to 50 ng/mL nicotine and 50 to 500 ng/mL cotinine. The method was validated on 150 plasma samples obtained from habitual smokers. Mean levels of 19.5 and 219 ng/mL were found for nicotine and cotinine, respectively. Both the mean and the range of the levels were in agreement with previously reported plasma levels for nicotine and cotinine.

Author Information

Kogan, MJ
Psychiatric Institute, New York, N.Y.
Verebey, K
, Brooklyn, N.Y.
Jaffee, JH
Psychiatric Institute, New York, N.Y.
Mulé, SJ
, Brooklyn, N.Y.
Pages: 6
Price: $25.00
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Stock #: JFS11324J
ISSN: 0022-1198
DOI: 10.1520/JFS11324J