ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 November 1987
Page Count: 7

The Use of Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels in the Characterization of Some Polymorphic Proteins of Forensic Science Significance
Alonso, A
Professors, Biology Section, National Institute of Toxicology,

Gascó, P
Professors, Biology Section, National Institute of Toxicology,

(Received 13 December 1986; accepted 11 February 1987)

Abstract

The identification of phenotypes of erythyrocyte acid phosphatase (EAP), esterase D (EsD), group specific component (Gc), and alpha-1-antitrypsin (PI) by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 45 mm) is described. The protein patterns obtained are compared favorably with the patterns seen by isoelectric focusing in conventional polyacrylamide gel dimensions (interelectrode distance: 110 to 120 mm). The technique described allows greater stability of pH gradients and is a fast and economic method.

Keywords:
pathology and biology, genetic typing, serology, paternity, protein polymorphism, separator isoelectric focusing, micro-ultrathin polyacrylamide gel, erythrocyte acid phosphatase, esterase D, group specific component, alpha-1-antitrypsin

Paper ID: JFS11215J
DOI: 10.1520/JFS11215J
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