Volume 32, Issue 6 (November 1987)
The Use of Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels in the Characterization of Some Polymorphic Proteins of Forensic Science Significance
The identification of phenotypes of erythyrocyte acid phosphatase (EAP), esterase D (EsD), group specific component (Gc), and alpha-1-antitrypsin (PI) by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 45 mm) is described. The protein patterns obtained are compared favorably with the patterns seen by isoelectric focusing in conventional polyacrylamide gel dimensions (interelectrode distance: 110 to 120 mm). The technique described allows greater stability of pH gradients and is a fast and economic method.