The Use of Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels in the Characterization of Some Polymorphic Proteins of Forensic Science Significance

    Volume 32, Issue 6 (November 1987)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 November 1987

    Page Count: 7


    Alonso, A
    Professors, Biology Section, National Institute of Toxicology, Madrid,

    Gascó, P
    Professors, Biology Section, National Institute of Toxicology, Madrid,

    (Received 13 December 1986; accepted 11 February 1987)

    Abstract

    The identification of phenotypes of erythyrocyte acid phosphatase (EAP), esterase D (EsD), group specific component (Gc), and alpha-1-antitrypsin (PI) by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 45 mm) is described. The protein patterns obtained are compared favorably with the patterns seen by isoelectric focusing in conventional polyacrylamide gel dimensions (interelectrode distance: 110 to 120 mm). The technique described allows greater stability of pH gradients and is a fast and economic method.


    Paper ID: JFS11215J

    DOI: 10.1520/JFS11215J

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    Title The Use of Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels in the Characterization of Some Polymorphic Proteins of Forensic Science Significance
    Symposium , 0000-00-00
    Committee E30