ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 November 1987
Page Count: 7

Detection of Le^a Substance in Saliva Stains by Enzyme-Linked Immunosorbent Assay (ELISA) Using Anti-Gum Arabic Serum
Suyama, H
Assistant professor, associate professor, forensic chemist, graduate student, and professor, Nagasaki University School of Medicine,

Nakasono, I
Assistant professor, associate professor, forensic chemist, graduate student, and professor, Nagasaki University School of Medicine,

Narita, K
Bachelor of surgery, Nagasaki City Hospital,

Iwasaki, M
Assistant professor, associate professor, forensic chemist, graduate student, and professor, Nagasaki University School of Medicine,

Ogata, M
Assistant professor, associate professor, forensic chemist, graduate student, and professor, Nagasaki University School of Medicine,

Kubo, S
Assistant professor, associate professor, forensic chemist, graduate student, and professor, Nagasaki University School of Medicine,

Fukae, T
Chief scientist, Forensic Science Laboratory, Nagasaki Prefectural Police Headquarters,

(Received 10 February 1987; accepted 13 April 1987)

Abstract

It is known that rabbit anti-gum arabic (GA) serum has cross-reactivity with Le^a antigen, and that, by using this cross-reactive anti-Le^a antibody, the presence of Le^a antigen in red blood cells and saliva can be demonstrated with accuracy. We have devised a rapid and highly sensitive method for detecting Le^a substance in human saliva by the enzyme-linked immunosorbent assay (ELISA) method using an anti-Le^a antibody isolated from anti-GA serum by affinity chromatography on Synsorb Le^a. The ELISA plate, coated with the specific anti-Le^a antibody, adsorbed the Le^a substance in saliva which was subsequently identified by adding enzyme labeled anti-Le^a IgG in that order. The method could detect the Le^a substance in Le(a+) saliva stains as small as 0.1 by 0.1 cm in size that had been stored at room temperature for three weeks and in Le(a+) saliva stains 0.7 by 0.7 cm in size that had been stored for ten years. This method seems to be useful for quantitative analyses of the Le^a substance in various body fluids.

Keywords:
pathology and biology, immunology, enzyme-linked immunosorbent assay, saliva, Lewis blood group system, gum arabic

Paper ID: JFS11214J
DOI: 10.1520/JFS11214J
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