ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 September 1987
Page Count: 7

Glyoxalase I Typing and Phosphoglucomutase-1 Subtyping of a Single Hair
Gambel, AM
Scientist, research chemist, and scientist, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, VA

Budowle, B
Scientist, research chemist, and scientist, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, VA

Terrell, L
Scientist, research chemist, and scientist, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, VA

(Received 15 August 1986; accepted 3 November 1986)

Abstract

A technique is described for the typing of glyoxalase I (GLO I) and the subtyping of phosphoglucomutase-1 (PGM-1) from the root sheath cells of a single forcibly removed hair. This procedure does not require sample preparation and does not alter the morphological characteristics of the hair. The combined discrimination probability (DP) of the two markers taken together is 0.90 for whites and 0.89 for blacks. GLO I can be typed after four weeks, and PGM-1 can be typed after eight to fifteen weeks in hairs maintained at room temperature. Hairs mounted with Permount® showed loss of enzyme activity and loss of band sharpness.

Keywords:
forensic science, hair, phosphoglucomutase, glyoxalase, electrophoresis, isoelectric focusing, sheath cell analysis

Paper ID: JFS11169J
DOI: 10.1520/JFS11169J
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