Glyoxalase I Typing and Phosphoglucomutase-1 Subtyping of a Single Hair

    Volume 32, Issue 5 (September 1987)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 September 1987

    Page Count: 7


    Gambel, AM
    Scientist, research chemist, and scientist, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA

    Budowle, B
    Scientist, research chemist, and scientist, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA

    Terrell, L
    Scientist, research chemist, and scientist, Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA

    (Received 15 August 1986; accepted 3 November 1986)

    Abstract

    A technique is described for the typing of glyoxalase I (GLO I) and the subtyping of phosphoglucomutase-1 (PGM-1) from the root sheath cells of a single forcibly removed hair. This procedure does not require sample preparation and does not alter the morphological characteristics of the hair. The combined discrimination probability (DP) of the two markers taken together is 0.90 for whites and 0.89 for blacks. GLO I can be typed after four weeks, and PGM-1 can be typed after eight to fifteen weeks in hairs maintained at room temperature. Hairs mounted with Permount® showed loss of enzyme activity and loss of band sharpness.


    Paper ID: JFS11169J

    DOI: 10.1520/JFS11169J

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    Author
    Title Glyoxalase I Typing and Phosphoglucomutase-1 Subtyping of a Single Hair
    Symposium , 0000-00-00
    Committee E30