Determination of ABO Blood Groups by Radioimmunoassay Using 125I-Protein A

    Volume 32, Issue 2 (March 1987)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 March 1987

    Page Count: 4


    Fukushima, H
    Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine, Nagano,

    Hasekura, H
    Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine, Nagano,

    Yonemura, I
    Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine, Nagano,

    Ota, M
    Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine, Nagano,

    Segawa, M
    Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine, Nagano,

    (Received 31 March 1986; accepted 19 May 1986)

    Abstract

    Since the crystallizable fragment (Fc) portion of the immunoglobulin G (IgG) molecule is the binding site of Protein A, a radioimmunoassay procedure using 125I-Protein A was developed for identification of the ABO blood groups. The isotope level bound to Group A, B, or AB red cells decreased with the dilution of anti-A or -B, respectively. After sensitization by anti-A plus B in Group O serum, the isotope bindings were observed in Groups A, B, and AB cells, while no significant radioactive count appeared in Group O cells. Furthermore, there was little significant isotope binding in both Group A and B red cells sensitized by the serum from Group A or B blood containing mainly IgM anti-A or -B. A radioimmunoassay using 125I-Protein A is an excellent method for identifying ABO blood groups.


    Paper ID: JFS11156J

    DOI: 10.1520/JFS11156J

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    Title Determination of ABO Blood Groups by Radioimmunoassay Using 125I-Protein A
    Symposium , 0000-00-00
    Committee E30