ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 March 1987
Page Count: 4

Determination of ABO Blood Groups by Radioimmunoassay Using ¹²⁵I-Protein A
Fukushima, H
Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine,

Hasekura, H
Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine,

Yonemura, I
Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine,

Ota, M
Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine,

Segawa, M
Associate professor, research fellow, instructor, instructor, and professor, Shinshu University School of Medicine,

(Received 31 March 1986; accepted 19 May 1986)

Abstract

Since the crystallizable fragment (Fc) portion of the immunoglobulin G (IgG) molecule is the binding site of Protein A, a radioimmunoassay procedure using ¹²⁵I-Protein A was developed for identification of the ABO blood groups. The isotope level bound to Group A, B, or AB red cells decreased with the dilution of anti-A or -B, respectively. After sensitization by anti-A plus B in Group O serum, the isotope bindings were observed in Groups A, B, and AB cells, while no significant radioactive count appeared in Group O cells. Furthermore, there was little significant isotope binding in both Group A and B red cells sensitized by the serum from Group A or B blood containing mainly IgM anti-A or -B. A radioimmunoassay using ¹²⁵I-Protein A is an excellent method for identifying ABO blood groups.

Keywords:
pathology and biology, blood, antigen systems, radioimmunoassay, 125I-Protein A, ABO blood group

Paper ID: JFS11156J
DOI: 10.1520/JFS11156J
ASTM International is a member of CrossRef.