ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 March 1987
Page Count: 4

A Rapid Screening Technique for the Detection of Spermatozoa
Fraysier, HD
Forensic chemist, Monroe County Public Safety Laboratory, NY

(Received 7 March 1986; accepted 1 May 1986)

Abstract

Phase contrast microscopy has been used for some time to search for and identify spermatozoa. An enhancement to the technique using xylene in conjunction with phase contrast microscopy is discussed. The method has been found to save time by allowing identification of spermatozoa with xylene-enhanced phase contrast microscopy in many cases that would have been unsuccessful using the normal “dry” phase contrast microscopy techniques. This eliminates a staining and reexamination step.

Keywords:
criminalistics, microscopy, spermatozoa, phase contrast microscopy, halation, xylene

Paper ID: JFS11155J
DOI: 10.1520/JFS11155J
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