ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 1986
Page Count: 6

The Use of Ultrathin-Layer Agarose Gels for Phenotyping Erythrocyte Acid Phosphatase by Isoelectric Focusing
Stolorow, MD
Forensic serologist and serology coordinator, Illinois Department of State Police, Bureau of Forensic Sciences, Training and Applications Laboratory, IL

Frank, WE
Forensic serologist and serology coordinator, Illinois Department of State Police, Bureau of Forensic Sciences, Training and Applications Laboratory, IL

(Received 1 October 1985; accepted 17 October 1985)

Abstract

A method is described for the use of ultrathin-layer agarose gels in phenotyping erythrocyte acid phosphatase (EAP) by isoelectric focusing (IEF). The results obtained using ultrathin-layer agarose gels are shown to be equally reliable and reproducible in comparison to established ultrathin-layer polyacrylamide gels. IEF of EAP on 0.168-mm agarose gels took place in 90 min using the LKB Multiphor system. The technique described allows for both time and cost efficient phenotyping of EAP.

Keywords:
forensic science, genetic typing, phosphatase, isoelectric focusing

Paper ID: JFS11117J
DOI: 10.1520/JFS11117J
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