ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 October 1985
Page Count: 5

An Agarose Gel Electrophoretic Method for Typing Phosphoglucomutase-1, Esterase D, or Glyoxalase I
Budowle, B
Research chemist, Forensic Science Research Group, Laboratory Division, FBI Academy, VA

(Received 29 September 1984; accepted 13 December 1984)

Abstract

A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Bloodstain extracts were absorbed into 1-mm-thick agarose gels via an application mask. The electrode wick distance was 12 cm and electrophoresis was carried out at 400 V at 6°C. The electrophoretic run times were 30 min for glyoxalase and 1 h for esterase D or phosphoglucomutase. This method is reliable and produces highly resolved band patterns. Additionally, the shorter separation times as a result of the increased voltage gradient permitted typing of more samples in a given time period compared with presently used methods. This technique requires little technical expertise and can be incorporated into the laboratory at a minimal cost.

Keywords:
forensic science, genetic typing, electrophoresis, phosphoglucomutase, esterase D, glyoxalase I, bloodstains, agarose gel electrophoresis, application mask

Paper ID: JFS11063J
DOI: 10.1520/JFS11063J
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