Volume 30, Issue 4 (October 1985)

    An Agarose Gel Electrophoretic Method for Typing Phosphoglucomutase-1, Esterase D, or Glyoxalase I

    (Received 29 September 1984; accepted 13 December 1984)

    Published Online: October

    CODEN: JFSOAD

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    Abstract

    A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Bloodstain extracts were absorbed into 1-mm-thick agarose gels via an application mask. The electrode wick distance was 12 cm and electrophoresis was carried out at 400 V at 6°C. The electrophoretic run times were 30 min for glyoxalase and 1 h for esterase D or phosphoglucomutase. This method is reliable and produces highly resolved band patterns. Additionally, the shorter separation times as a result of the increased voltage gradient permitted typing of more samples in a given time period compared with presently used methods. This technique requires little technical expertise and can be incorporated into the laboratory at a minimal cost.


    Author Information:

    Budowle, B
    Research chemist, Forensic Science Research Group, Laboratory Division, FBI Academy, Quantico, VA


    Stock #: JFS11063J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11063J

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    Title An Agarose Gel Electrophoretic Method for Typing Phosphoglucomutase-1, Esterase D, or Glyoxalase I
    Symposium , 0000-00-00
    Committee E30