Volume 30, Issue 4 (October 1985)

    Identification of Fetal Bloodstains by Enzyme-Linked Immunosorbent Assay for Human α-Fetoprotein

    (Received 16 August 1984; accepted 4 January 1985)

    Published Online: October

    CODEN: JFSOAD

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    Abstract

    A rapid and highly sensitive enzyme-linked immunosorbent assay (ELISA) for determination of human α-fetoprotein (AFP) using commercially available reagents was devised and applied to identification of fetal bloodstains. When experimentally prepared bloodstains, 1 by 2 mm in area, were submitted to analysis, only fetal bloodstains showed positive reactions in the present ELISA. The reactions did not change significantly when these bloodstains were stored at room temperature for one week. The present ELISA seems to be suitable for forensic science practice.


    Author Information:

    Tsutsumi, H
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Tamaki, K
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Oya, M
    Professor, Yamanashi University School of Medicine, Yamanashi,

    Sato, M
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Yada, S
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,


    Stock #: JFS11062J

    ISSN: 0022-1198

    DOI: 10.1520/JFS11062J

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    Title Identification of Fetal Bloodstains by Enzyme-Linked Immunosorbent Assay for Human α-Fetoprotein
    Symposium , 0000-00-00
    Committee E30