Identification of Fetal Bloodstains by Enzyme-Linked Immunosorbent Assay for Human α-Fetoprotein

    Volume 30, Issue 4 (October 1985)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 October 1985

    Page Count: 6


    Tsutsumi, H
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Tamaki, K
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Oya, M
    Professor, Yamanashi University School of Medicine, Yamanashi,

    Sato, M
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Yada, S
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate professor, forensic chemist, graduate student, forensic chemist, and professor, Nagoya University School of Medicine, Nagoya,

    (Received 16 August 1984; accepted 4 January 1985)

    Abstract

    A rapid and highly sensitive enzyme-linked immunosorbent assay (ELISA) for determination of human α-fetoprotein (AFP) using commercially available reagents was devised and applied to identification of fetal bloodstains. When experimentally prepared bloodstains, 1 by 2 mm in area, were submitted to analysis, only fetal bloodstains showed positive reactions in the present ELISA. The reactions did not change significantly when these bloodstains were stored at room temperature for one week. The present ELISA seems to be suitable for forensic science practice.


    Paper ID: JFS11062J

    DOI: 10.1520/JFS11062J

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    Title Identification of Fetal Bloodstains by Enzyme-Linked Immunosorbent Assay for Human α-Fetoprotein
    Symposium , 0000-00-00
    Committee E30