ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 1985
Page Count: 5

Discontinuous Polyacrylamide Gel Electrophoresis for Typing Haptoglobin in Bloodstains
Budowle, B
Research chemist and student intern, Forensic Science Research Group, Laboratory Division, FBI Academy, VA

Chow, GH
Research chemist and student intern, Forensic Science Research Group, Laboratory Division, FBI Academy, VA

(Received 22 September 1984; accepted 9 November 1984)

Abstract

A routine method is described for obtaining reproducible haptoglobin patterns from bloodstains by discontinuous polyacrylamide gel electrophoresis. By employing a stacking gel, proteins from bloodstain extracts are concentrated into narrow zones, before entering the resolving gel. This effect yields highly resolved haptoglobin patterns. Therefore, laboratories without the specialized equipment and expertise for polyacrylamide gradient gel preparation can still obtain highly resolved haptoglobin patterns from bloodstains.

Keywords:
pathology and biology, haptoglobin, blood, bloodstains, discontinuous polyacrylamide gel, electrophoresis

Paper ID: JFS11022J
DOI: 10.1520/JFS11022J
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