Discontinuous Polyacrylamide Gel Electrophoresis for Typing Haptoglobin in Bloodstains

    Volume 30, Issue 3 (July 1985)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 1985

    Page Count: 5


    Budowle, B
    Research chemist and student intern, Forensic Science Research Group, Laboratory Division, FBI Academy, Quantico, VA

    Chow, GH
    Research chemist and student intern, Forensic Science Research Group, Laboratory Division, FBI Academy, Quantico, VA

    (Received 22 September 1984; accepted 9 November 1984)

    Abstract

    A routine method is described for obtaining reproducible haptoglobin patterns from bloodstains by discontinuous polyacrylamide gel electrophoresis. By employing a stacking gel, proteins from bloodstain extracts are concentrated into narrow zones, before entering the resolving gel. This effect yields highly resolved haptoglobin patterns. Therefore, laboratories without the specialized equipment and expertise for polyacrylamide gradient gel preparation can still obtain highly resolved haptoglobin patterns from bloodstains.


    Paper ID: JFS11022J

    DOI: 10.1520/JFS11022J

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    Title Discontinuous Polyacrylamide Gel Electrophoresis for Typing Haptoglobin in Bloodstains
    Symposium , 0000-00-00
    Committee E30