ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 1985
Page Count: 23

Evaluation of Antisera for Bloodstain Grouping I. ABH, MN, and Rh
Pagliaro, EM
Chief criminalist and director, and senior criminalist, State Police Forensic Science Laboratory, CT

Lee, HC
Chief criminalist and director, and senior criminalist, State Police Forensic Science Laboratory, CT

Bremser, JK
Professor and director, and senior research associate, Forensic Science Program, University of New Haven, CT

Gaensslen, RE
Professor and director, and senior research associate, Forensic Science Program, University of New Haven, CT

(Received 9 July 1984; accepted 24 November 1984)

Abstract

Sixty-eight different commercially available blood grouping antisera and lectins with ABH, MN, and Rh D, C, E, c, and e specificities were serologically evaluated for their applicability to bloodstain antigen determination. The characteristics of the antisera were determined with red cells, with fresh bloodstains, and with series of aging bloodstains. The Rh antisera were tested under a variety of serological conditions and with bloodstains on various substrata. Additionally, studies on optimization of absorption-elution procedure variables were carried out, and some data on the storage characteristics of red cells and blood grouping antisera were gathered.

Keywords:
pathology and biology, genetic typing, antigen systems, blood groups, bloodstains, ABO system, MN system, Rh system, absorption-elution, antiserum evaluation

Paper ID: JFS10999J
DOI: 10.1520/JFS10999J
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