An Evaluation of Gamma Glutamyl Transpeptidase (GGT) and p30 Determinations for the Identification of Semen on Postcoital Vaginal Swabs

Volume 30, Issue 3 (July 1985)

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ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 1985
Page Count: 11

An Evaluation of Gamma-Glutamyl Transpeptidase (GGT) and p30 Determinations for the Identification of Semen on Postcoital Vaginal Swabs
Stubbings, NA
Forensic analyst and forensic research, Centre of Forensic Sciences, Ontario

Newall, PJ
Forensic analyst and forensic research, Centre of Forensic Sciences, Ontario

(Received 4 September 1984; accepted 7 February 1985)

Abstract

The following study entails the investigation of gamma-glutamyl transpeptidase (GGT) and p30 for the identification of seminal stains in sexual assault cases. A commercial kit was used to test for GGT activity, while p30 was demonstrated with a crossed electrophoresis technique. Specificity, sensitivity, and stability of both markers were studied. Postcoital swabs from lab staff were tested for GGT and p30. In addition, 144 postcoital swabs from case material were tested for p30, spermatozoa, and acid phosphatase. Results show p30 to be a useful semen marker particularly in cases of azoospermia. However, GGT was found to be unsuitable for forensic science casework.

Keywords:
criminalistics, criminal sex offenses, semen, postcoital vaginal swabs

Paper ID: JFS10997J
DOI: 10.1520/JFS10997J
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Author Title An Evaluation of Gamma-Glutamyl Transpeptidase (GGT) and p30 Determinations for the Identification of Semen on Postcoital Vaginal Swabs Symposium , 0000-00-00 Committee E30

		SEDL / Journals / Journal of Forensic Sciences (JOFS) / Citation Page Volume 30, Issue 3 (July 1985) Click here to download this paper now for $25 PDF (408K) View License Agreement ISSN: 0022-1198 CODEN: JFSCA Published Online: 1 July 1985 Page Count: 11 An Evaluation of Gamma-Glutamyl Transpeptidase (GGT) and p30 Determinations for the Identification of Semen on Postcoital Vaginal Swabs Stubbings, NA Forensic analyst and forensic research, Centre of Forensic Sciences, Ontario Newall, PJ Forensic analyst and forensic research, Centre of Forensic Sciences, Ontario (Received 4 September 1984; accepted 7 February 1985) Abstract The following study entails the investigation of gamma-glutamyl transpeptidase (GGT) and p30 for the identification of seminal stains in sexual assault cases. A commercial kit was used to test for GGT activity, while p30 was demonstrated with a crossed electrophoresis technique. Specificity, sensitivity, and stability of both markers were studied. Postcoital swabs from lab staff were tested for GGT and p30. In addition, 144 postcoital swabs from case material were tested for p30, spermatozoa, and acid phosphatase. Results show p30 to be a useful semen marker particularly in cases of azoospermia. However, GGT was found to be unsuitable for forensic science casework. Keywords: criminalistics, criminal sex offenses, semen, postcoital vaginal swabs Paper ID: JFS10997J DOI: 10.1520/JFS10997J ASTM International is a member of CrossRef. Author Title An Evaluation of Gamma-Glutamyl Transpeptidase (GGT) and p30 Determinations for the Identification of Semen on Postcoital Vaginal Swabs Symposium , 0000-00-00 Committee E30