Enzyme Assays for the Identification of Gastric Fluid

    Volume 30, Issue 1 (January 1985)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1985

    Page Count: 6


    Galvin, C
    Chief medical examiner, State of Connecticut, Office of Chief Medical Examiner, Farmington, CT

    Pagliaro, EM
    senior criminalist, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Lee, HC
    Chief criminalist and director, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Gaensslen, RE
    Professor and director of forensic science, University of New Haven, West Haven, CT

    (Received 21 May 1984; accepted 8 June 1984)

    Abstract

    Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories. These samples are usually vomitus, or stomach contents originating from wounds that perforate the stomach. They may be encountered at scenes or on articles submitted for examination, in fresh form or as dried stains. The pepsin activity assay is based on proteolytic activity with bovine albumin as substrate and the rennin-like activity assay is based on the coagulation of milk protein.


    Paper ID: JFS10969J

    DOI: 10.1520/JFS10969J

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    Title Enzyme Assays for the Identification of Gastric Fluid
    Symposium , 0000-00-00
    Committee E30