Volume 30, Issue 1 (January 1985)

    Enzyme Assays for the Identification of Gastric Fluid

    (Received 21 May 1984; accepted 8 June 1984)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories. These samples are usually vomitus, or stomach contents originating from wounds that perforate the stomach. They may be encountered at scenes or on articles submitted for examination, in fresh form or as dried stains. The pepsin activity assay is based on proteolytic activity with bovine albumin as substrate and the rennin-like activity assay is based on the coagulation of milk protein.


    Author Information:

    Galvin, C
    Chief medical examiner, State of Connecticut, Office of Chief Medical Examiner, Farmington, CT

    Pagliaro, EM
    senior criminalist, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Lee, HC
    Chief criminalist and director, Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Gaensslen, RE
    Professor and director of forensic science, University of New Haven, West Haven, CT


    Stock #: JFS10969J

    ISSN: 0022-1198

    DOI: 10.1520/JFS10969J

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    Author
    Title Enzyme Assays for the Identification of Gastric Fluid
    Symposium , 0000-00-00
    Committee E30