Volume 25, Issue 1 (January 1980)

    The Simultaneous Identification of Seminal Acid Phosphatase and Phosphoglucomutase by Starch Gel Electrophoresis

    (Received 26 October 1978; accepted 30 May 1979)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.


    Author Information:

    Linde, HG
    Chemist-criminalist, Vermont State Police Laboratory, Montpelier,

    Molnar, KE
    Criminalist II, BCI East Central Laboratory, Cambridge, Ohio


    Stock #: JFS10945J

    ISSN: 0022-1198

    DOI: 10.1520/JFS10945J

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    Title The Simultaneous Identification of Seminal Acid Phosphatase and Phosphoglucomutase by Starch Gel Electrophoresis
    Symposium , 0000-00-00
    Committee E30