ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 1980
Page Count: 5

The Simultaneous Identification of Seminal Acid Phosphatase and Phosphoglucomutase by Starch Gel Electrophoresis
Linde, HG
Chemist-criminalist, Vermont State Police Laboratory,

Molnar, KE
Criminalist II, BCI East Central Laboratory, Ohio

(Received 26 October 1978; accepted 30 May 1979)

Abstract

Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.

Keywords:
pathology and biology, phosphatases, phosphoglucomutase, electrophoresis

Paper ID: JFS10945J
DOI: 10.1520/JFS10945J
ASTM International is a member of CrossRef.