(Received 26 October 1978; accepted 30 May 1979)
Published Online: January
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Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.
Chemist-criminalist, Vermont State Police Laboratory, Montpelier,
Criminalist II, BCI East Central Laboratory, Cambridge, Ohio
Stock #: JFS10945J