ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 1980
Page Count: 4

A New Enzymatic Method for the Demonstration of Spermine in Human Seminal Stains
Oya, M
Associate professor, research associate, and professor (director), Nagoya University School of Medicine,

Suzuki, O
Senior lecturer, Hamamatsu University School of Medicine,

Yada, S
Associate professor, research associate, and professor (director), Nagoya University School of Medicine,

Matsumoto, T
Research associate, Nagoya University School of Medicine,

Katsumata, Y
Associate professor, research associate, and professor (director), Nagoya University School of Medicine,

(Received 28 March 1979; accepted 21 June 1979)

Abstract

A new, simple method for the identification of seminal stains based on the reaction of bovine plasma amine oxidase (spermine oxidase) with spermine is described. The key reaction is the production of hydrogen peroxide by the oxidase action on spermine; the hydrogen peroxide oxidizes 2',7'-dichlorofluoresein to produce a light green color by which the presence of seminal fluid can be identified. Some data are presented on the stability and specificity of the present method.

Keywords:
pathology and biology, semen, chemical analysis

Paper ID: JFS10943J
DOI: 10.1520/JFS10943J
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