A New Enzymatic Method for the Demonstration of Spermine in Human Seminal Stains

    Volume 25, Issue 1 (January 1980)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1980

    Page Count: 4


    Oya, M
    Associate professor, research associate, and professor (director), Nagoya University School of Medicine, Nagoya,

    Suzuki, O
    Senior lecturer, Hamamatsu University School of Medicine, Hamamatsu,

    Yada, S
    Associate professor, research associate, and professor (director), Nagoya University School of Medicine, Nagoya,

    Matsumoto, T
    Research associate, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate professor, research associate, and professor (director), Nagoya University School of Medicine, Nagoya,

    (Received 28 March 1979; accepted 21 June 1979)

    Abstract

    A new, simple method for the identification of seminal stains based on the reaction of bovine plasma amine oxidase (spermine oxidase) with spermine is described. The key reaction is the production of hydrogen peroxide by the oxidase action on spermine; the hydrogen peroxide oxidizes 2',7'-dichlorofluoresein to produce a light green color by which the presence of seminal fluid can be identified. Some data are presented on the stability and specificity of the present method.


    Paper ID: JFS10943J

    DOI: 10.1520/JFS10943J

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    Title A New Enzymatic Method for the Demonstration of Spermine in Human Seminal Stains
    Symposium , 0000-00-00
    Committee E30