ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 1980
Page Count: 4

The Preparation of Lipid-Free Extracts of Drugs from Blood for Gas Chromatographic Analysis
Freitag, J
Toxicologists, Hamilton County Coroner's Office, Ohio

Tye, R
Toxicologists, Hamilton County Coroner's Office, Ohio

(Received 10 March 1979; accepted 28 May 1979)

Abstract

A method is presented to separate co-extracted biological materials, in particular lipids, from drugs in chloroform extracts of blood. The chloroform is evaporated and the residue is dissolved in a small volume of petroleum ether and partitioned with eight times this volume of 5% aqueous hydrochloric acid. The acidic, basic, and neutral drugs are extracted into the aqueous phase, leaving unwanted materials in the petroleum ether. The recoveries of drugs representative of a variety of types are presented. Reduction of the levels of lipids by factors on the order of 1000-fold is reported.

Keywords:
toxicology, lipids, chromatographic analysis

Paper ID: JFS10942J
DOI: 10.1520/JFS10942J
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