The Preparation of Lipid-Free Extracts of Drugs from Blood for Gas Chromatographic Analysis

    Volume 25, Issue 1 (January 1980)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1980

    Page Count: 4


    Freitag, J
    Toxicologists, Hamilton County Coroner's Office, Cincinnati, Ohio

    Tye, R
    Toxicologists, Hamilton County Coroner's Office, Cincinnati, Ohio

    (Received 10 March 1979; accepted 28 May 1979)

    Abstract

    A method is presented to separate co-extracted biological materials, in particular lipids, from drugs in chloroform extracts of blood. The chloroform is evaporated and the residue is dissolved in a small volume of petroleum ether and partitioned with eight times this volume of 5% aqueous hydrochloric acid. The acidic, basic, and neutral drugs are extracted into the aqueous phase, leaving unwanted materials in the petroleum ether. The recoveries of drugs representative of a variety of types are presented. Reduction of the levels of lipids by factors on the order of 1000-fold is reported.


    Paper ID: JFS10942J

    DOI: 10.1520/JFS10942J

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    Title The Preparation of Lipid-Free Extracts of Drugs from Blood for Gas Chromatographic Analysis
    Symposium , 0000-00-00
    Committee E30