Volume 25, Issue 1 (January 1980)

    Determination of Phencyclidine by Radioimmunoassay

    (Received 2 March 1979; accepted 28 May 1979)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    A radioimmunoassay (RIA) was developed for measurement of phencyclidine (PCP) and its monohydroxy metabolites in urine. Anti-PCP serum was generated in rabbits and the labeled antigen was prepared by radio-iodination (125I) by the Hunter-Greenwood procedure. Adjustment of the antiserum and 125I-antigen concentrations resulted in a dynamic response from 0 to 200 ng/ml and as little as 2 ng/ml was detected. Reagents were stable for at least three months at 2 to 8° Most (95%) urine specimens from presumed nonusers contained less than 5 ng/ml and urine specimens from suspected abusers usually exceeded 100 ng/ml. Comparison of RIA with other methods for detection of PCP showed good agreement. Cross-reactivity with other commonly prescribed drugs was not encountered. The recovery of PCP was 93 to 109% over the 20 to 175 ng/ml concentration range and the reproducibility (average coefficient of variation) was ±13%. Development of the RIA for PCP has resulted in a rapid procedure that can be adapted to automated processing and is also suitable for small-scale testing.


    Author Information:

    Ward, C
    Senior research group chief, associate scientist, and assistant scientist, Hoffmann-La Roche, Inc., Nutley, N.J.

    Anthony, M
    Senior research group chief, associate scientist, and assistant scientist, Hoffmann-La Roche, Inc., Nutley, N.J.

    Heveran, ZE
    Senior research group chief, associate scientist, and assistant scientist, Hoffmann-La Roche, Inc., Nutley, N.J.


    Stock #: JFS10940J

    ISSN: 0022-1198

    DOI: 10.1520/JFS10940J

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    Author
    Title Determination of Phencyclidine by Radioimmunoassay
    Symposium , 0000-00-00
    Committee E30