The Screening and Quantitation of Diazepam, Flurazepam, Chlordiazepoxide, and Their Metabolites in Blood and Plasma by Electron-Capture Gas Chromatography and High Pressure Liquid Chromatography

    Volume 24, Issue 1 (January 1979)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1979

    Page Count: 9


    Kopjak, L
    Associate toxicologists, Center for Human Toxicology, University of Utah, Salt Lake City,

    Peat, MA
    Associate toxicologists, Center for Human Toxicology, University of Utah, Salt Lake City,

    (Received 11 March 1978; accepted 1 May 1978)

    Abstract

    The number and frequency of requests made to toxicologists to analyze blood or plasma samples for benzodiazepines are increasing dramatically, but until recently specific and sensitive procedures were unavailable to meet the demand. Gas-liquid chromatography with electron-capture detection has become the analytical method of choice for these drugs because it meets the necessary sensitivity requirements. Prior to the development of this method the available analytical procedures depended on acid hydrolysis of the benzodiazepines to their corresponding benzophenones [1] followed by gas-liquid or thin-layer chromatography. Unfortunately, hydrolysis of several of the benzodiazepines results in the formation of the same benzophenone [2,3] so that the individual parent drugs cannot be readily identified.


    Paper ID: JFS10788J

    DOI: 10.1520/JFS10788J

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    Title The Screening and Quantitation of Diazepam, Flurazepam, Chlordiazepoxide, and Their Metabolites in Blood and Plasma by Electron-Capture Gas Chromatography and High Pressure Liquid Chromatography
    Symposium , 0000-00-00
    Committee E30