Volume 23, Issue 2 (April 1978)

    Determination of Group Specific Component Phenotypes in Dried Bloodstains by Immunofixation on Cellulose Acetate

    (Received 1 July 1977; accepted 3 August 1977)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    A rapid method is described for the determination of the genetically controlled group specific component (Gc) system. The method uses electrophoresis on cellulose acetate membranes followed by immunofixation with a specific anti-Gc antiserum. The method has been successfully applied to samples of plasma, whole blood, and dried bloodstains. Multiple specimens, up to 16 samples, can be analyzed simultaneously in a total analysis time of less than 60 min. In contrast to conventional immunoelectrophoresis on agar gel, or immunofixation on agarose or starch gel [1], this method of immunofixation on cellulose acetate is rapid, reliable, and economical.


    Author Information:

    Grunbaum, BW
    Criminalist, and research biochemist and consultant, Criminalistics Laboratory, Pleasanton, Calif.

    Zajac, PL
    Criminalist, and research biochemist and consultant, Criminalistics Laboratory, Pleasanton, Calif.


    Stock #: JFS10767J

    ISSN: 0022-1198

    DOI: 10.1520/JFS10767J

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    Title Determination of Group Specific Component Phenotypes in Dried Bloodstains by Immunofixation on Cellulose Acetate
    Symposium , 0000-00-00
    Committee E30