ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 October 1978
Page Count: 3

Phase Contrast Microscopy Versus Differential Interference Contrast Microscopy as Applicable to the Observation of Spermatozoa
Boudreau, AJ
Criminalist, Fresno County Sheriffs Department Crime Laboratory, Calif.

Cortner, GV
Criminalist, Fresno Regional Criminalistics Laboratory, Calif.

(Received 13 December 1977; accepted 17 April 1978)

Abstract

A review of available literature has shown something of a paucity of suggested and applied microscopic techniques on the identification of human spermatozoa in forensic casework. Phase contrast microscopy (PC) has been mentioned as the desirable technique by Kirk [1] and Kivela [2]. The advantages of PC as compared with bright-field are well known and would most certainly include simplifying the task of locating any spermatozoa present. Some other advantages are an apparent improvement in the morphological detail presented to the observer and some reduction in the fatigue associated with the eyestrain accompanying this frequently tedious task.

Keywords:

Paper ID: JFS10744J
DOI: 10.1520/JFS10744J
ASTM International is a member of CrossRef.