Detection of Barbiturates by Latex Agglutination Inhibition

    Volume 23, Issue 3 (July 1978)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 July 1978

    Page Count: 11


    Tonchen, A
    Research group chief, assistant scientist, and associate scientist, Hoffmann-La Roche, Inc., Nutley, N.J.

    Bergamini, JA
    Associate scientist, Hoffmann-La Roche, Inc., Belleville, N.J.

    Heveran, JE
    Research group chief, assistant scientist, and associate scientist, Hoffmann-La Roche, Inc., Nutley, N.J.

    Cox, M
    Research group chief, assistant scientist, and associate scientist, Hoffmann-La Roche, Inc., Nutley, N.J.

    (Received 28 October 1977; accepted 12 December 1977)

    Abstract

    Spector and Flynn [1] initially demonstrated that antibodies to barbiturates could be elicited by immunization of rabbits with a secobarbital derivative coupled to bovine serum albumin. The resultant antiserum and a 14C- or 125I-labeled barbiturate derivative were used to develop a sensitive and specific radioimmunoassay [1–3]. Although the radio-immunoassay obviated the need for any sample pretreatment, it did require a nuclear counter and the use of radioactive materials. An enzyme-coupled immunoassay [4] and a hemagglutination inhibition method [5] have been reported as alternative approaches for the detection of barbiturates.


    Paper ID: JFS10693J

    DOI: 10.1520/JFS10693J

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    Title Detection of Barbiturates by Latex Agglutination Inhibition
    Symposium , 0000-00-00
    Committee E30