ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 July 1978
Page Count: 11

Detection of Barbiturates by Latex Agglutination Inhibition
Tonchen, A
Research group chief, assistant scientist, and associate scientist, Hoffmann-La Roche, Inc., N.J.

Bergamini, JA
Associate scientist, Hoffmann-La Roche, Inc., N.J.

Heveran, JE
Research group chief, assistant scientist, and associate scientist, Hoffmann-La Roche, Inc., N.J.

Cox, M
Research group chief, assistant scientist, and associate scientist, Hoffmann-La Roche, Inc., N.J.

(Received 28 October 1977; accepted 12 December 1977)

Abstract

Spector and Flynn [1] initially demonstrated that antibodies to barbiturates could be elicited by immunization of rabbits with a secobarbital derivative coupled to bovine serum albumin. The resultant antiserum and a ¹⁴C- or ¹²⁵I-labeled barbiturate derivative were used to develop a sensitive and specific radioimmunoassay [1–3]. Although the radio-immunoassay obviated the need for any sample pretreatment, it did require a nuclear counter and the use of radioactive materials. An enzyme-coupled immunoassay [4] and a hemagglutination inhibition method [5] have been reported as alternative approaches for the detection of barbiturates.

Keywords:

Paper ID: JFS10693J
DOI: 10.1520/JFS10693J
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