Volume 23, Issue 3 (July 1978)
Detection of Barbiturates by Latex Agglutination Inhibition
Spector and Flynn  initially demonstrated that antibodies to barbiturates could be elicited by immunization of rabbits with a secobarbital derivative coupled to bovine serum albumin. The resultant antiserum and a 14C- or 125I-labeled barbiturate derivative were used to develop a sensitive and specific radioimmunoassay [1–3]. Although the radio-immunoassay obviated the need for any sample pretreatment, it did require a nuclear counter and the use of radioactive materials. An enzyme-coupled immunoassay  and a hemagglutination inhibition method  have been reported as alternative approaches for the detection of barbiturates.