Journal Published Online: 01 July 1978
Volume 23, Issue 3

Detection of Barbiturates by Latex Agglutination Inhibition

CODEN: JFSCAS

Abstract

Spector and Flynn [1] initially demonstrated that antibodies to barbiturates could be elicited by immunization of rabbits with a secobarbital derivative coupled to bovine serum albumin. The resultant antiserum and a 14C- or 125I-labeled barbiturate derivative were used to develop a sensitive and specific radioimmunoassay [1–3]. Although the radio-immunoassay obviated the need for any sample pretreatment, it did require a nuclear counter and the use of radioactive materials. An enzyme-coupled immunoassay [4] and a hemagglutination inhibition method [5] have been reported as alternative approaches for the detection of barbiturates.

Author Information

Heveran, JE
Hoffmann-La Roche, Inc., Nutley, N.J.
Cox, M
Hoffmann-La Roche, Inc., Nutley, N.J.
Tonchen, A
Hoffmann-La Roche, Inc., Nutley, N.J.
Bergamini, JA
Hoffmann-La Roche, Inc., Belleville, N.J.
Pages: 11
Price: $25.00
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Stock #: JFS10693J
ISSN: 0022-1198
DOI: 10.1520/JFS10693J